Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq.
Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq.
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通过libla-seq在康复covid-19患者中的SARS-COV-2特异性记忆B细胞的快速分离和免疫分析。
DOI：
10.1038/s41392-021-00610-7
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发表时间：
2021-05-17
影响因子：
39.3
通讯作者：
Chen YQ
中科院分区：
文献类型：
--
作者：
He B;Liu S;Wang Y;Xu M;Cai W;Liu J;Bai W;Ye S;Ma Y;Hu H;Meng H;Sun T;Li Y;Luo H;Shi M;Du X;Zhao W;Chen S;Yang J;Zhu H;Jie Y;Yang Y;Guo D;Wang Q;Liu Y;Yan H;Wang M;Chen YQ
B细胞应答在抗SARS-CoV-2感染中起关键作用。然而，对SARS-CoV-2感染后配对的SARS-CoV-2抗原特异性BCR库的多样性和频率知之甚少。在这里，我们使用从5名恢复期COVID-19患者中分离的记忆和血浆B细胞进行了单细胞RNA测序和VDJ测序，并分析了抗体免疫反应的谱和转录异质性。通过测序将BCR与抗原特异性连接（LIBRA-seq），我们鉴定了一种独特的激活的记忆B细胞亚群（CD 11 chigh CD 95 high），其SARS-CoV-2抗原标记细胞的比例高于记忆B细胞。我们的研究结果揭示了配对BCR库的多样性和SARS-CoV-2感染后SARS-CoV-2抗原特异性免疫球蛋白重链和轻链的非随机配对。不同的恢复期个体共享公共抗体克隆型。此外，通过ELISA测定，通过LIBRA-seq分离的几种抗体显示出对SARS-CoV-2受体结合结构域（RBD）或核蛋白（NP）的高结合亲和力。两个RBD反应性抗体C14646 P3 S和C2767 P3 S通过LIBRA-seq分离显示出高中和活性的假型和真实的SARS-CoV-2病毒在体外。我们的研究提供了基本的见解B细胞反应后，SARS-CoV-2感染在单细胞水平。
B cell response plays a critical role against SARS-CoV-2 infection. However, little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection. Here, we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients, and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses. Via linking BCR to antigen specificity through sequencing (LIBRA-seq), we identified a distinct activated memory B cell subgroup (CD11chigh CD95high) had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells. Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection. The public antibody clonotypes were shared by distinct convalescent individuals. Moreover, several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain (RBD) or nucleoprotein (NP) via ELISA assay. Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro. Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level.
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影响因子：
64.5
作者：
Alt FW;Zhang Y;Meng FL;Guo C;Schwer B
通讯作者：
Schwer B
影响因子：
30.3
作者：
Jackson KJ;Liu Y;Roskin KM;Glanville J;Hoh RA;Seo K;Marshall EL;Gurley TC;Moody MA;Haynes BF;Walter EB;Liao HX;Albrecht RA;García-Sastre A;Chaparro-Riggers J;Rajpal A;Pons J;Simen BB;Hanczaruk B;Dekker CL;Laserson J;Koller D;Davis MM;Fire AZ;Boyd SD
通讯作者：
Boyd SD
DOI：
10.1093/bioinformatics/bts565
发表时间：
2012-12-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Fu L;Niu B;Zhu Z;Wu S;Li W
通讯作者：
Li W
DOI：
10.1111/j.1749-6632.2009.04813.x
发表时间：
2009-01-01
期刊：
CONTEMPORARY CHALLENGES IN AUTOIMMUNITY
影响因子：
--
作者：
Lacotte, Stephanie;Brun, Susana;Dumortier, Helene
通讯作者：
Dumortier, Helene
影响因子：
32.4
作者：
Mesin, Luka;Ersching, Jonatan;Victora, Gabriel D.
通讯作者：
Victora, Gabriel D.