Human responses to influenza vaccination show seroconversion signatures and convergent antibody rearrangements.
Human responses to influenza vaccination show seroconversion signatures and convergent antibody rearrangements.
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DOI：
10.1016/j.chom.2014.05.013
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发表时间：
2014-07-09
影响因子：
30.3
通讯作者：
Boyd SD
中科院分区：
文献类型：
--
作者：
Jackson KJ;Liu Y;Roskin KM;Glanville J;Hoh RA;Seo K;Marshall EL;Gurley TC;Moody MA;Haynes BF;Walter EB;Liao HX;Albrecht RA;García-Sastre A;Chaparro-Riggers J;Rajpal A;Pons J;Simen BB;Hanczaruk B;Dekker CL;Laserson J;Koller D;Davis MM;Fire AZ;Boyd SD
B细胞通过基因重排产生不同的抗体库。病原体暴露可诱导表达能结合病原体的抗体的B细胞的克隆性增殖。为了评估人类B细胞对三价季节性流感和单价大流行H1N1疫苗的反应，我们对编码免疫球蛋白重链的基因重排进行了测序，免疫球蛋白重链是表位识别的主要决定因素。B细胞克隆扩增的大小与个体对疫苗的分泌抗体反应有关，扩增的克隆对那些表达流感特异性单抗的克隆是丰富的。此外，B细胞对大流行性流感H1N1疫苗接种和不同人群感染的反应显示出针对流感抗原的聚集性抗体重链基因重排的显著家族。这些结果表明，微生物可以诱导免疫球蛋白基因重排的特定特征，病原体暴露可以从B细胞谱系进行潜在的评估。
B cells produce a diverse antibody repertoire by undergoing gene rearrangements. Pathogen exposure induces the clonal expansion of B cells expressing antibodies that can bind the infectious agent. To assess human B cell responses to trivalent seasonal influenza and monovalent pandemic H1N1 vaccination, we sequenced gene rearrangements encoding the immunoglobulin heavy chain, a major determinant of epitope recognition. The magnitude of B cell clonal expansions correlates with an individual’s secreted antibody response to the vaccine and the expanded clones are enriched for those expressing influenza-specific mAbs. Additionally, B cell responses to pandemic influenza H1N1 vaccination and infection in different people show a prominent family of convergent antibody heavy chain gene rearrangements specific to influenza antigens. These results indicate that microbes can induce specific signatures of immunoglobulin gene rearrangements and that pathogen exposure can potentially be assessed from B cell repertoires.
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DOI：
10.4049/jimmunol.1101823
发表时间：
2011-10-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Krause JC;Tsibane T;Tumpey TM;Huffman CJ;Briney BS;Smith SA;Basler CF;Crowe JE Jr
通讯作者：
Crowe JE Jr
影响因子：
17.1
作者：
Jiang N;He J;Weinstein JA;Penland L;Sasaki S;He XS;Dekker CL;Zheng NY;Huang M;Sullivan M;Wilson PC;Greenberg HB;Davis MM;Fisher DS;Quake SR
通讯作者：
Quake SR
影响因子：
64.8
作者：
Wrammert, Jens;Smith, Kenneth;Wilson, Patrick C.
通讯作者：
Wilson, Patrick C.
影响因子：
5.4
作者：
Sasaki, Sanae;Jaimes, Maria C.;Greenberg, Harry B.
通讯作者：
Greenberg, Harry B.
影响因子：
6.4
作者：
BROKSTAD, KA;COX, RJ;LARS, R
通讯作者：
LARS, R