Spatial encoded polarization dependent nonlinear optical analysis for local tensors imaging of collagenous tissue
Spatial encoded polarization dependent nonlinear optical analysis for local tensors imaging of collagenous tissue
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用于胶原组织局部张量成像的空间编码偏振相关非线性光学分析
DOI：
10.1117/12.2510049
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Wilson, Tony
中科院分区：
文献类型：
--
作者：
Ding, Changqin;Ulcickas, James R.;Deng, Fengyuan;Simpson, Garth;Brown, Thomas G.;Wilson, Tony
利用空间编码偏振相关非线性光学测量方法实现了胶原组织的快速局部超极化率张量成像。二次谐波（Secondharmonic generation，SHG）由于其独特的对称性要求，对偏振相关测量非常敏感，为蛋白质晶体和生物组织提供了丰富的局部结构信息。快速偏振相关测量可降低1/f噪声并抑制体内成像的运动模糊。在这项工作中，空间编码的偏振相关的二次谐波用于局部超极化张量成像的z切石英和胶原组织通过使用一个单一的图案化的微延迟器阵列（μRA）。μRA被设计成具有在快轴的方位角方向上空间变化的半波延迟图案。当放置在光束扫描显微镜的后共轭平面时，μRA能够对入射光进行空间调制，使视场中不同位置的偏振态发生变化。“快照”方法可用于均匀样品的偏振相关测量，使得一个图像包括来自不同像素的完整偏振调制集。结合样本平移，该方法能够恢复非均匀样本的局部超极化率张量。该策略成功地用于提取z切石英和胶原组织的局部非线性光学张量，与传统的偏振相关测量具有良好的一致性，为胶原组织的快速偏振分析提供了一种替代方法，对当前光束扫描非线性光学系统进行了最小的修改。
Rapid local hyperpolarizability tensor imaging of collagenous tissue was achieved with spatially encoded polarization dependent nonlinear optical measurements. Second harmonic generation (SHG) is sensitive to polarization-dependent measurements due to its unique symmetry requirement, providing rich information of local structures for protein crystals and biological tissues. Fast polarization-dependent measurements reduce 1/f noise and suppress motion blur for in vivo imaging. In this work, spatially encoded polarization dependent SHG was used for local hyperpolarizability tensor imaging of z-cut quartz and collagenous tissue by using a single patterned microretarder array (μRA). The μRA was designed with a pattern of half-wave retardance varying spatially in the azimuthal orientation of the fast-axis. When placed in the rear conjugate plane of a beam scanning microscope, the μRA enabled spatial modulation of incident light with polarization states varied at different positions in the field of view. The ‘snapshot’ approach was available for the polarization dependent measurements of a uniform sample so that one image included a complete set of polarization modulation from different pixels. Combining with sample translation, this method was able to recover local hyperpolarizability tensor of non-uniform samples. This strategy was successfully used to extract local nonlinear optical tensors for z-cut quartz and collagenous tissue with good agreements with traditional polarization dependent measurements, providing an alternate approach for fast polarization analysis of collagen tissue with minimal modifications on current beam scanning nonlinear optical systems.
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DOI：
10.1364/josab.17.001685
发表时间：
2000-10-01
影响因子：
1.9
作者：
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通讯作者：
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影响因子：
3.5
作者：
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影响因子：
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作者：
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