A rapid and massive gene expression shift marking adolescent transition in C. elegans.
A rapid and massive gene expression shift marking adolescent transition in C. elegans.
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DOI：
10.1038/srep03912
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发表时间：
2014-01-28
影响因子：
4.6
通讯作者：
Kammenga JE
中科院分区：
文献类型：
--
作者：
Snoek LB;Sterken MG;Volkers RJ;Klatter M;Bosman KJ;Bevers RP;Riksen JA;Smant G;Cossins AR;Kammenga JE
器官发育是生命周期中最具活力的时期，但我们对青春期过渡期基因表达的动态只有粗略的了解。在这里，我们表明，青春期的秀丽隐杆线虫的特点是一个壮观的表达转变的保守和高度多态性的基因。使用高分辨率的时间序列，我们发现在青春期蠕虫中，超过10，000个基因改变了它们的表达。这些基因根据其表达模式进行聚类。一个簇涉及染色质重塑显示出短暂的上调约50小时后孵化。 与此同时，观察到了一个惊人的表情变化。这个集群在许多基因型的序列比较显示多样化的选择。强烈上调的基因在非编码区显示出纯化选择的迹象，表明具有生物活性的基因受到其调控特性的限制。我们的研究结果提高了我们对青春期过渡的理解，并有助于消除由于不正确的发育时机而造成的实验假象。
Organismal development is the most dynamic period of the life cycle, yet we have only a rough understanding of the dynamics of gene expression during adolescent transition. Here we show that adolescence in Caenorhabditis elegans is characterized by a spectacular expression shift of conserved and highly polymorphic genes. Using a high resolution time series we found that in adolescent worms over 10,000 genes changed their expression. These genes were clustered according to their expression patterns. One cluster involved in chromatin remodelling showed a brief up-regulation around 50 h post-hatch. At the same time a spectacular shift in expression was observed. Sequence comparisons for this cluster across many genotypes revealed diversifying selection. Strongly up-regulated genes showed signs of purifying selection in non-coding regions, indicating that adolescence-active genes are constrained on their regulatory properties. Our findings improve our understanding of adolescent transition and help to eliminate experimental artefacts due to incorrect developmental timing.
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影响因子：
16
作者：
Reinke, V;Smith, HE;Kim, SK
通讯作者：
Kim, SK
影响因子：
30.8
作者：
Andersen, Erik C.;Gerke, Justin P.;Shapiro, Joshua A.;Crissman, Jonathan R.;Ghosh, Rajarshi;Bloom, Joshua S.;Felix, Marie-Anne;Kruglyak, Leonid
通讯作者：
Kruglyak, Leonid
影响因子：
4.5
作者：
Coolon JD;Jones KL;Todd TC;Carr BC;Herman MA
通讯作者：
Herman MA
影响因子：
5.8
作者：
Hibbs, Matthew A.;Hess, David C.;Troyanskaya, Olga G.
通讯作者：
Troyanskaya, Olga G.
影响因子：
3.3
作者：
Cutter, AD
通讯作者：
Cutter, AD