The application of eDNA for monitoring of the Great Crested Newt in the UK.
The application of eDNA for monitoring of the Great Crested Newt in the UK.
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DOI：
10.1002/ece3.1272
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发表时间：
2014-11
影响因子：
2.6
通讯作者：
Gough, Kevin C.
中科院分区：
文献类型：
--
作者：
Rees, Helen C.;Bishop, Keith;Middleditch, David J.;Patmore, James R. M.;Maddison, Ben C.;Gough, Kevin C.
目前对大冠蝾螈的生态调查既耗时又昂贵，而且只能在很短的调查窗口内进行。额外的调查方法将有助于发现稀有或受保护的物种，如大冠蝾螈（Triturus cristatus）。环境DNA （eDNA）分析已被用于丹麦大冠蝾螈的检测。在这里，同样的方法被应用于从英国池塘采集的水样，同时使用传统的实地测量技术。我们的eDNA分析显示出84%的成功率，现场和eDNA调查之间的kappa一致系数为0.86。一个通过现场调查确定为阴性的大冠蝾螈池塘通过eDNA分析呈阳性，表明使用该方法可以提高检出率。夏末采集的水样分析表明，在现有野外技术的最佳调查窗口过后，eDNA分析可以用于检测大冠蝾螈。因此，eDNA分析可以增加目前规定的大冠蝾螈调查技术，作为一种相对快速和廉价的工具，在英国收集大冠蝾螈的存在和分布数据，而不是在全面的实地调查之前。
Current ecological surveys for great crested newts are time-consuming and expensive and can only be carried out within a short survey window. Additional survey methods which would facilitate the detection of rare or protected species such as the great crested newt (Triturus cristatus) would be extremely advantageous. Environmental DNA (eDNA) analysis has been utilized for the detection of great crested newts in Denmark. Here, the same methodology has been applied to water samples taken from UK ponds concurrently with conventional field surveying techniques. Our eDNA analysis exhibited an 84% success rate with a kappa coefficient of agreement between field and eDNA surveys of 0.86. One pond determined to be negative for great crested newt by field survey was positive by eDNA analysis, revealing the potential for improved detection rates using this methodology. Analysis of water samples collected in late summer indicates that eDNA analysis could be used to detect great crested newt after the optimal survey window for current field techniques had passed. Consequently, eDNA analysis could augment currently stipulated techniques for great crested newt surveying as a relatively quick and inexpensive tool for collecting great crested newt presence and distribution data within the UK instead of or prior to full field surveys.
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