DASH transcription factor impacts Medicago truncatula seed size by its action on embryo morphogenesis and auxin homeostasis.
DASH transcription factor impacts Medicago truncatula seed size by its action on embryo morphogenesis and auxin homeostasis.
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DOI：
10.1111/tpj.12742
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发表时间：
2015-02
期刊：
影响因子：
--
通讯作者：
Verdier J
中科院分区：
文献类型：
--
作者：
Noguero M;Le Signor C;Vernoud V;Bandyopadhyay K;Sanchez M;Fu C;Torres-Jerez I;Wen J;Mysore KS;Gallardo K;Udvardi M;Thompson R;Verdier J
胚乳在控制种子发育的分子信号组成组织之间的整合中起着关键作用。它已被证明参与胚胎形态发生的调节，并最终决定种子大小。然而，调节种子大小的分子机制仍然知之甚少，特别是在豆类。DASH（DOF Acting in Seed embryogenesis and Hormone accumulation）是一种在蒺藜苜蓿种子合点胚乳中表达的DOF转录因子。三个独立的破折号突变体等位基因的表型特征揭示了这种TF在防止早期种子败育和最终种子大小的确定中的作用。强功能丧失等位基因导致胚乳发育严重缺陷，并导致胚生长停滞在球形阶段。Dash豆荚与野生型（WT）豆荚的转录组学分析揭示了主要的转录变化，并突出显示了参与生长素运输和感知的基因，主要是在Dash突变豆荚中表达不足。有趣的是，外源生长素的应用减轻了种子致死表型，而激素剂量显示了更高的生长素含量在短跑豆荚相比，野生型。总之，这些结果表明，生长素的运输/信号可能会受到影响的破折号突变体和异常生长素分布可能会导致胚胎发生的缺陷，最终导致种子大小表型。
The endosperm plays a pivotal role in the integration between component tissues of molecular signals controlling seed development. It has been shown to participate in the regulation of embryo morphogenesis and ultimately seed size determination. However, the molecular mechanisms that modulate seed size are still poorly understood especially in legumes. DASH (DOF Acting in Seed embryogenesis and Hormone accumulation) is a DOF transcription factor (TF) expressed during embryogenesis in the chalazal endosperm of the Medicago truncatula seed. Phenotypic characterization of three independent dash mutant alleles revealed a role for this TF in the prevention of early seed abortion and the determination of final seed size. Strong loss-of-function alleles cause severe defects in endosperm development and lead to embryo growth arrest at the globular stage. Transcriptomic analysis of dash pods versus wild-type (WT) pods revealed major transcriptional changes and highlighted genes that are involved in auxin transport and perception as mainly under-expressed in dash mutant pods. Interestingly, the exogenous application of auxin alleviated the seed-lethal phenotype, whereas hormonal dosage revealed a much higher auxin content in dash pods compared with WT. Together these results suggested that auxin transport/signaling may be affected in the dash mutant and that aberrant auxin distribution may contribute to the defect in embryogenesis resulting in the final seed size phenotype.
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影响因子：
7.4
作者：
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通讯作者：
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DOI：
10.1073/pnas.260502697
发表时间：
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影响因子：
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影响因子：
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作者：
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