Normalized Quantitative PCR Measurements as Predictors for Ethene Formation at Sites Impacted with Chlorinated Ethenes.
Normalized Quantitative PCR Measurements as Predictors for Ethene Formation at Sites Impacted with Chlorinated Ethenes.
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DOI：
10.1021/acs.est.8b04373
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发表时间：
2018-11-20
影响因子：
11.4
通讯作者：
Löffler FE
中科院分区：
文献类型：
--
作者：
Clark K;Taggart DM;Baldwin BR;Ritalahti KM;Murdoch RW;Hatt JK;Löffler FE
针对麦卡脱卤球菌（Dhc）生物标志物基因的定量PCR（qPCR）支持在受氯化乙烯影响的地点进行有效管理。为了建立Dhc生物标志物基因丰度和乙烯形成之间的相关性（即，解毒），859个地下水样品，代表62个地点进行监测自然衰减或加强补救进行了分析。在含有乙烯的威尔斯孔中，分别有88%和61%的样本检测到Dhc 16 S rRNA基因和氯乙烯（VC）还原脱卤酶基因bvcA和vcrA。Dhc 16 S rRNA、bvcA、vcrA和tceA（参与共代谢还原VC脱氯）基因丰度均与乙烯形成呈正相关。当Dhc 16 S rRNA基因和VC RDase基因丰度分别超过107和106拷贝L−1时，以及当Dhc 16 S rRNA-和bvcA + vcrA-与总细菌16 S rRNA基因比率超过0.1%时，观察到乙烯浓度显著更高。Dhc 16 S rRNA基因与vcrA/bvcA的比值接近1也表明乙烯升高;然而，在vcrA和/或bvcA基因拷贝数超过Dhc细胞数10- 10 000倍的19个威尔斯孔中未观察到乙烯增加。大约三分之一的可检测到乙烯的样品缺乏bvcA，vcrA和tceA，这表明尚未实现对VC解毒生物标志物的全面了解。虽然目前的生物标志物套件是不完整的，数据分析证实了现有的Dhc DNA生物标志物的预测和诊断地下水监测受氯化乙烯影响的网站的价值。
Quantitative PCR (qPCR) targeting Dehalococcoides mccartyi (Dhc) biomarker genes supports effective management at sites impacted with chlorinated ethenes. To establish correlations between Dhc biomarker gene abundances and ethene formation (i.e., detoxification), 859 groundwater samples representing 62 sites undergoing monitored natural attenuation or enhanced remediation were analyzed. Dhc 16S rRNA genes and the vinyl chloride (VC) reductive dehalogenase genes bvcA and vcrA were detected in 88% and 61% of samples, respectively, from wells with ethene. Dhc 16S rRNA, bvcA, vcrA, and tceA (implicated in cometabolic reductive VC dechlorination) gene abundances all positively correlated with ethene formation. Significantly greater ethene concentrations were observed when Dhc 16S rRNA gene and VC RDase gene abundances exceeded 107 and 106 copies L−1, respectively, and when Dhc 16S rRNA- and bvcA + vcrA-to-total bacterial 16S rRNA gene ratios exceeded 0.1%. Dhc 16S rRNA gene-to-vcrA/bvcA ratios near unity also indicated elevated ethene; however, no increased ethene was observed in 19 wells where vcrA and/or bvcA gene copy numbers exceeded Dhc cell numbers 10- to 10 000-fold. Approximately one-third of samples with detectable ethene lacked bvcA, vcrA, and tceA, suggesting that comprehensive understanding of VC detoxification biomarkers has not been achieved. Although the current biomarker suite is incomplete, the data analysis corroborates the value of the available Dhc DNA biomarkers for prognostic and diagnostic groundwater monitoring at sites impacted with chlorinated ethenes.
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影响因子：
3.7
作者：
Clooney AG;Fouhy F;Sleator RD;O' Driscoll A;Stanton C;Cotter PD;Claesson MJ
通讯作者：
Claesson MJ
影响因子：
4.4
作者：
Krajmalnik-Brown, R;Hölscher, T;Löffler, FE
通讯作者：
Löffler, FE
DOI：
10.1007/978-1-4614-4115-1_2
发表时间：
2013-01-01
期刊：
BIOAUGMENTATION FOR GROUNDWATER REMEDIATION
影响因子：
--
作者：
Loeffler, Frank E.;Ritalahti, Kirsti M.;Zinder, Stephen H.
通讯作者：
Zinder, Stephen H.
影响因子：
5.1
作者：
He, J;Sung, Y;Löffler, FE
通讯作者：
Löffler, FE
影响因子：
4.4
作者：
Cheng, Dan;He, Jianzhong
通讯作者：
He, Jianzhong