Functional gene-guided discovery of type II polyketides from culturable actinomycetes associated with soft coral Scleronephthya sp.
Functional gene-guided discovery of type II polyketides from culturable actinomycetes associated with soft coral Scleronephthya sp.
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功能基因引导下从与软珊瑚硬肾病相关的可培养放线菌中发现 II 型聚酮化合物
DOI：
10.1371/journal.pone.0042847
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Sun W;Peng C;Zhao Y;Li Z
与石珊瑚中的放线菌相比，软珊瑚相关的可培养放线菌的系统发育多样性基本上尚未被探索。同时，对珊瑚相关放线菌天然产物的了解非常有限。在这项研究中，从软珊瑚 Scleronephthya sp. 的组织中分离出 32 株菌株。通过16S rDNA系统发育分析将其分为8个属：小单孢菌属、戈尔登菌属、分枝杆菌属、诺卡氏菌属、链霉菌属、纤维单胞菌属、饮食菌属和红球菌属。通过对PKS II（II型聚酮化合物合酶）基因簇中KSα（酮脂酰合酶）基因的分析，发现6个小单孢菌菌株和4个链霉菌菌株具有生产芳香族聚酮化合物的潜力。在6个小单孢菌菌株中，2个菌株（A5-1和A6-2）扩增了安古环素环化酶基因，表明它们在合成安古环素等方面具有潜力。贾多霉素。在功能基因预测指导下，在小单孢菌发酵液中检测到了一种贾多霉素B类似物（7b，13-二氢-7-O-甲基贾多霉素B）。菌株A5-1。这项研究强调了与软珊瑚 Scleronephthya sp. 组织相关的系统发育多样性的可培养放线菌。以及珊瑚源放线菌尤其是小单孢菌在生产芳香族聚酮化合物方面的潜力。
Compared with the actinomycetes in stone corals, the phylogenetic diversity of soft coral-associated culturable actinomycetes is essentially unexplored. Meanwhile, the knowledge of the natural products from coral-associated actinomycetes is very limited. In this study, thirty-two strains were isolated from the tissue of the soft coral Scleronephthya sp. in the East China Sea, which were grouped into eight genera by 16S rDNA phylogenetic analysis: Micromonospora, Gordonia, Mycobacterium, Nocardioides, Streptomyces, Cellulomonas, Dietzia and Rhodococcus. 6 Micromonospora strains and 4 Streptomyces strains were found to be with the potential for producing aromatic polyketides based on the analysis of KSα (ketoacyl-synthase) gene in the PKS II (type II polyketides synthase) gene cluster. Among the 6 Micromonospora strains, angucycline cyclase gene was amplified in 2 strains (A5-1 and A6-2), suggesting their potential in synthesizing angucyclines e.g. jadomycin. Under the guidance of functional gene prediction, one jadomycin B analogue (7b, 13-dihydro-7-O-methyl jadomycin B) was detected in the fermentation broth of Micromonospora sp. strain A5-1. This study highlights the phylogenetically diverse culturable actinomycetes associated with the tissue of soft coral Scleronephthya sp. and the potential of coral-derived actinomycetes especially Micromonospora in producing aromatic polyketides.
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影响因子：
4.2
作者：
Nithyanand, Paramasivam;Pandian, Shunmugiah Karutha
通讯作者：
Pandian, Shunmugiah Karutha
海南软珊瑚 Scleronephthya gracillimum Kukenthal 的新孕烷类似物
影响因子：
1.6
作者：
Han, Lei;Wang, Chang-Yun;Gu, Yu-Cheng
通讯作者：
Gu, Yu-Cheng
影响因子：
5.1
作者：
Khan, Shams Tabrez;Komaki, Hisayuki;Shin-ya, Kazuo
通讯作者：
Shin-ya, Kazuo
影响因子：
4.4
作者：
Mincer, TJ;Jensen, PR;Fenical, W
通讯作者：
Fenical, W
影响因子：
3.3
作者：
Baz, JP;Canedo, LM;Elipe, MVS
通讯作者：
Elipe, MVS