In vivo visualization of reactive gliosis using manganese-enhanced magnetic resonance imaging.
In vivo visualization of reactive gliosis using manganese-enhanced magnetic resonance imaging.
复制标题
使用锰增强的磁共振成像对反应性神经胶质病的体内可视化。
DOI：
10.1016/j.neuroimage.2009.11.005
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发表时间：
2010-02-15
期刊：
影响因子：
5.7
通讯作者：
Tanaka C
中科院分区：
文献类型：
--
作者：
Kawai Y;Aoki I;Umeda M;Higuchi T;Kershaw J;Higuchi M;Silva AC;Tanaka C
反应性星形胶质细胞增生发生在各种中枢神经系统（CNS）损伤后。虽然星形胶质细胞增生提供了对炎症的保护，但它也阻碍了CNS损伤后神经再生的进展。因此，一种能够在体内可视化和组织表征神经胶质增生的方法对于CNS损伤和相应治疗的研究将是非常宝贵的。锰已被证明是一种有用的MRI造影剂，通过Ca2+通道进入细胞，并已被应用于锰增强MRI（MEMRI）神经元功能映射。本研究旨在探讨MEMRI是否可以检测体内局灶性缺血后的星形胶质细胞增生。根据短暂性大脑中动脉闭塞或假手术后的天数将大鼠分组。MEMRI的环形或新月形增强对应于大脑中动脉闭塞后11天在缺血核心的外周区域观察到的GFAP阳性星形胶质细胞。这表明MEMRI增强主要反映了卒中后的反应性星形胶质细胞增生。
Reactive astrogliosis occurs after diverse central nervous system (CNS) insults. While astrogliosis provides protection against inflammation, it is also obstructive in the progress of neuranagenesis after CNS insults. Thus, a method that enables in vivo visualization and tissue characterization for gliosis would be invaluable for studies of CNS insults and corresponding treatments. Manganese has proven to be a useful MRI contrast agent that enters cells via Ca2+ channels and has been applied to manganese-enhanced MRI (MEMRI) for neuronal functional mapping. This study investigated whether MEMRI can detect astrogliosis after focal ischemia in vivo. Rats were divided into groups according to the number of days after either transient middle cerebral artery occlusion or a sham. Ring- or crescent-shaped enhancement of MEMRI corresponded to the GFAP-positive astroglia observed in the peripheral region of the ischemic core 11 days after middle cerebral artery occlusion. This indicates that MEMRI enhancement predominantly reflects reactive astrogliosis after stroke.
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