Derivation of cranial neural crest-like cells from human embryonic stem cells.
Derivation of cranial neural crest-like cells from human embryonic stem cells.
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DOI：
10.1016/j.bbrc.2008.09.032
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发表时间：
2008-11-21
影响因子：
3.1
通讯作者：
Snead, Malcolm L.
中科院分区：
文献类型：
--
作者：
Zhou, Yan;Snead, Malcolm L.
神经嵴是一个多能祖细胞的短暂群体，在整个脊椎动物胚胎中形成各种组织。胚胎干细胞（ES）能够形成胚状体并自发分化为各种谱系，遵循可重复的时间发育模式，重现早期胚胎发生。将拟胚体研磨，并将解离的细胞进行荧光激活细胞分选（FACS）处理，并且超过1%的细胞被鉴定为卷曲-3 +/钙粘蛋白-11+。在软骨细胞、神经胶质、神经元、成骨细胞和平滑肌中检测到与各种终末命运相关的标记基因的表达，表明FACS分选的卷曲蛋白-3 +/钙粘蛋白-11+细胞是能够分化为与颅神经嵴相关的命运的多能祖细胞。此外，分选的细胞能够自我更新并保持多能分化潜能。从ES细胞中分离获得颅神经嵴样多能祖细胞，为体外神经嵴细胞谱系分析提供了新的工具。
The neural crest is a transient population of multipotent progenitors contributing to a diverse array of tissues throughout the vertebrate embryo. Embryonic stem (ES) cells are able to form embryoid body and spontaneously differentiate to various lineages, following a reproducible temporal pattern of development that recapitulates early embryogenesis. Embryoid bodies were triturated and the dissociated cells were processed for fluorescence-activated cell sorting (FACS), and more than 1% of cells were identified as frizzled-3+/cadherin-11+. Expression of marker genes associated with various terminal fates was detected for chondrocytes, glia, neurons, osteoblasts and smooth muscles, indicating that the FACS-sorted frizzled-3+/cadherin-11+ cells were multipotent progenitor cells capable of differentiating to fates associated with cranial neural crest. Moreover, the sorted cells were able to self-renew and maintain multipotent differentiation potential. The derivation of cranial neural crest-like multipotent progenitor cells from ES cells provides a new tool for cell lineage analysis of neural crest in vitro.
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影响因子：
2.7
作者：
KIMURA, Y;MATSUNAMI, H;TAKEICHI, M
通讯作者：
TAKEICHI, M
影响因子：
64.8
作者：
Bhanot, P;Brink, M;Nusse, R
通讯作者：
Nusse, R
影响因子：
2.6
作者：
Borello, U;Buffa, V;Cossu, G
通讯作者：
Cossu, G
DOI：
10.1083/jcb.200411095
发表时间：
2005-04-25
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kléber M;Lee HY;Wurdak H;Buchstaller J;Riccomagno MM;Ittner LM;Suter U;Epstein DJ;Sommer L
通讯作者：
Sommer L
影响因子：
15.9
作者：
Kehat, I;Kenyagin-Karsenti, D;Gepstein, L
通讯作者：
Gepstein, L