Effects of n-3 PUFAs on Intestinal Mucosa Innate Immunity and Intestinal Microbiota in Mice after Hemorrhagic Shock Resuscitation.
Effects of n-3 PUFAs on Intestinal Mucosa Innate Immunity and Intestinal Microbiota in Mice after Hemorrhagic Shock Resuscitation.
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作者：
Tian F;Gao X;Zhang L;Wang X;Wan X;Jiang T;Wu C;Bi J;Lei Q
n-3 多不饱和脂肪酸 (PUFA) 可以改善缺血再灌注或失血性休克复苏 (HSR) 损伤后的肠道屏障功能。然而，n-3 PUFA 对 HSR 后肠道微生物群和肠粘膜先天免疫的影响仍不清楚。在本研究中，40 只 C57BL/6J 小鼠被随机分为五组：对照组、假手术组、HSR、HSR + n-3 PUFA 和 HSR + n-6 PUFA。 HSR 后 12 小时处死小鼠。收集肝脏、脾脏、肠系膜淋巴结和回肠末端组织。无菌刮除肠粘膜。与 HSR 组相比，PUFA 给药组的杯状细胞数量增加，粘蛋白 2 的表达恢复，扰乱的肠道微生物群部分稳定，表明 n-3 和 n-6 PUFA 均减少了γ-变形菌的过度增殖，同时促进了拟杆菌的生长。值得注意的是，n-3 PUFA 在改善 HSR 后回肠组织溶菌酶水平方面比 n-6 PUFA 具有优势。因此，PUFA，尤其是n-3 PUFA，部分改善了HSR后小鼠肠粘膜的先天免疫。这些发现表明了向缺血再灌注恢复的患者提供 n-3 PUFA 的临床原理。
n-3 polyunsaturated fatty acids (PUFAs) can improve the function of the intestinal barrier after damage from ischemia-reperfusion or hemorrhagic shock resuscitation (HSR). However, the effects of n-3 PUFAs on intestinal microbiota and the innate immunity of the intestinal mucosa after HSR remain unclear. In the present study, 40 C57BL/6J mice were randomly assigned to five groups: control, sham, HSR, HSR + n-3 PUFAs and HSR + n-6 PUFAs. Mice were sacrificed 12 h after HSR. Liver, spleen, mesenteric lymph nodes and terminal ileal tissues were collected. Intestinal mucosae were scraped aseptically. Compared with the HSR group, the number of goblet cells increased, expression of mucin 2 was restored and disturbed intestinal microbiota were partly stabilized in the PUFA-administered groups, indicating that both n-3 and n-6 PUFAs reduced overproliferation of Gammaproteobacteria while promoting the growth of Bacteroidetes. Notably, n-3 PUFAs had an advantage over n-6 PUFAs in improving ileal tissue levels of lysozyme after HSR. Thus, PUFAs, especially n-3 PUFAs, partly improved the innate immunity of intestinal mucosa in mice after HSR. These findings suggest a clinical rationale for providing n-3 PUFAs to patients recovering from ischemia-reperfusion.
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影响因子：
8.8
作者：
Grau-Carmona, Teodoro;Bonet-Saris, Alfonso;Mesejo, Alfonso
通讯作者：
Mesejo, Alfonso
DOI：
10.1186/s13054-015-0888-7
发表时间：
2015-04-16
期刊：
Critical care (London, England)
影响因子：
--
作者：
Manzanares W;Langlois PL;Dhaliwal R;Lemieux M;Heyland DK
通讯作者：
Heyland DK
DOI：
10.1186/cc13850
发表时间：
2014-04-29
期刊：
Critical care (London, England)
影响因子：
--
作者：
Hecker M;Ott J;Sondermann C;Schaefer M;Obert M;Hecker A;Morty RE;Vadasz I;Herold S;Rosengarten B;Witzenrath M;Seeger W;Mayer K
通讯作者：
Mayer K
影响因子：
5.9
作者：
Juman S;Hashimoto M;Katakura M;Inoue T;Tanabe Y;Arita M;Miki T;Shido O
通讯作者：
Shido O
影响因子：
24.5
作者：
Grootjans, Joep;Hundscheid, Inca H. R.;Buurman, Wim A.
通讯作者：
Buurman, Wim A.