Cultivable Winogradskyella species are genomically distinct from the sympatric abundant candidate species.
Cultivable Winogradskyella species are genomically distinct from the sympatric abundant candidate species.
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可栽培的 Winogradskyella 物种在基因组上有别于同域的大量候选物种。
DOI：
10.1038/s43705-021-00052-w
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发表时间：
2021-09-29
期刊：
影响因子：
--
通讯作者：
Amann, Rudolf
中科院分区：
文献类型：
--
作者：
Alejandre-Colomo, Carlota;Francis, Ben;Viver, Tomeu;Harder, Jens;Fuchs, Bernhard M.;Rossello-Mora, Ramon;Amann, Rudolf
Winogradsky是拟杆菌门中的一个属，具有明确的海洋起源。该属的大多数成员已被发现与海洋动物和藻类有关，但也与沙子等无机表面有关。在这项研究中，我们分析了最近从北海表层海水样本中分离出的11个物种的基因组，这些物种在一次春季藻类水华期间。相应的后生基因组为该属产生了一个念珠菌属种。除了W.ursingii外，所有培养的物种都隶属于Winogradsky ella谱系，其特征是基因组大(~4.3 ± 0.4 Mb)，其碳水化合物和蛋白质降解基因具有高度的复杂性。具体来说，每个菌株的多糖利用位点(Puls)是不同的，这表明底物的多样性很大。虽然这些菌株存在于北海，但它们的丰度达到或低于后基因组的检测下限。与之相反，该单一种被归类为Candidatus W.Atlantica，所有北海的Mags都属于该物种，属于一个谱系，其中栽培的代表显示出~3.03.5 Mb的小基因组，Mags有~2.3Mb的 。在加利福尼亚州基因组流线化明显导致精氨酸、蛋氨酸、亮氨酸和缬氨酸等多种氨基酸的生物合成途径丧失，PUL基因座因利用海带多糖而减少到单一的一个。这种尚未培育的物种似乎利用了藻类水华释放的零星丰富的底物，主要是海带多糖。我们还认为，这种精简的基因组可能是这种念珠菌物种缺乏平板生长的原因，而不是数量较少但共存的该属成员的生长。
Winogradskyella is a genus within the phylum Bacteroidetes with a clear marine origin. Most members of this genus have been found associated with marine animals and algae, but also with inorganic surfaces such as sand. In this study, we analyzed genomes of eleven species recently isolated from surface seawater samples from the North Sea during a single spring algae bloom. Corresponding metagenomes yielded a single Candidatus species for this genus. All species in culture, with the exception of W. ursingii, affiliated with a Winogradskyella lineage characterized by large genomes (~4.3 ± 0.4 Mb), with high complexity in their carbohydrate and protein degradation genes. Specifically, the polysaccharide utilization loci (PULs) were diverse within each individual strain, indicating large substrate versatility. Although present in the North Sea, the abundances of these strains were at, or below, the detection limit of the metagenomes. In contrast, the single species, classified as Candidatus W. atlantica, to which all North Sea MAGs belonged, affiliated with a lineage in which the cultivated representatives showed small genomes of ~3.0–3.5 Mb, with the MAGs having ~2.3 Mb. In Ca. W. atlantica, genome streamlining has apparently resulted in the loss of biosynthesis pathways for several amino acids including arginine, methionine, leucine and valine, and the PUL loci were reduced to a single one for utilizing laminarin. This as-yet uncultivated species seems to capitalize on sporadically abundant substrates that are released by algae blooms, mainly laminarin. We also suggest that this streamlined genome might be responsible for the lack of growth on plates for this Candidatus species, in contrast to growth of the less abundant but coexisting members of the genus.
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影响因子：
5.1
作者：
Hahnke, Richard L.;Bennke, Christin M.;Harder, Jens
通讯作者：
Harder, Jens
影响因子：
6.4
作者：
Grote J;Thrash JC;Huggett MJ;Landry ZC;Carini P;Giovannoni SJ;Rappé MS
通讯作者：
Rappé MS
DOI：
10.1073/pnas.1917001117
发表时间：
2020-03-24
影响因子：
11.1
作者：
Becker, Stefan;Tebben, Jan;Hehemann, Jan-Hendrik
通讯作者：
Hehemann, Jan-Hendrik
影响因子：
5.1
作者：
Bauer, Margarete;Kube, Michael;Gloeckner, Frank Oliver
通讯作者：
Gloeckner, Frank Oliver
影响因子：
11
作者：
Carini, Paul;Steindler, Laura;Giovannoni, Stephen J.
通讯作者：
Giovannoni, Stephen J.