Expression Patterns of Developmental Control Genes in Normal andEngrailed-1 Mutant Mouse Spinal Cord Reveal Early Diversity in Developing Interneurons
Expression Patterns of Developmental Control Genes in Normal andEngrailed-1 Mutant Mouse Spinal Cord Reveal Early Diversity in Developing Interneurons
复制标题
正常和Engrailed-1突变小鼠脊髓中发育控制基因的表达模式揭示了发育中神经元的早期多样性
作者：
M. Matise;A. Joyner
脊椎动物脊髓长期以来一直是研究沿背腹 (d/v) 轴的细胞分化模式的有用系统。在本文中，我们定义了在小鼠脊髓中间区（IR）的限制性 d/v 结构域中表达的几类基因的表达，其中产生了大多数中间神经元。通过该分析，我们发现脊髓中间神经元及其前体在分化开始时表达转录因子和Notch配体的独特组合。许多不同类别基因的表达域具有相似的边界，表明腹侧IR可能基本细分为四个不同的区域。这种差异基因表达表明脊髓中间神经元在发育早期获得独特的身份，并且 Notch 信号传导机制可能参与沿 d/v 轴的细胞命运的决定。 Engrailed-1 (En-1) 突变体中的基因表达研究表明，表达 En-1 的神经元和其他位置紧密的神经元类别不需要同源域蛋白 En-1 来实现其早期分化模式。相反，有人认为 En-1 可能在脊髓后期成熟过程中发挥区分中间神经元子集的作用。
The vertebrate spinal cord has long served as a useful system for studying the pattern of cell differentiation along the dorsoventral (d/v) axis. In this paper, we have defined the expression of several classes of genes expressed in restricted d/v domains in the intermediate region (IR) of the mouse spinal cord, in which most interneurons are generated. From this analysis, we have found that spinal cord interneurons and their precursors express unique combinations of transcription factors and Notch ligands at the onset of their differentiation. The domains of expression of a number of different classes of genes share similar boundaries, indicating that there could be a basic subdivision of the ventral IR into four distinct regions. This differential gene expression suggests that spinal cord interneurons acquire unique identities early in their development and that Notch signaling mechanisms may participate in the determination of cell fate along the d/v axis. Gene expression studies inEngrailed-1 (En-1) mutants showed thatEn-1-expressing and other closely positioned classes of neurons do not require the homeodomain protein En-1 for their early pattern of differentiation. Rather, it is suggested thatEn-1 may function to distinguish a subset of interneurons during the later maturation of the spinal cord.
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