Silencing the HaAK gene by transgenic plant-mediated RNAi impairs larval growth of Helicoverpa armigera.
Silencing the HaAK gene by transgenic plant-mediated RNAi impairs larval growth of Helicoverpa armigera.
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通过转基因植物介导的 RNAi 沉默 HaAK 基因会损害棉铃虫幼虫的生长
DOI：
10.7150/ijbs.10468
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发表时间：
2015
影响因子：
9.2
通讯作者：
Sun J
中科院分区：
文献类型：
--
作者：
Liu F;Wang XD;Zhao YY;Li YJ;Liu YC;Sun J
害虫给农业造成了巨大的经济损失，而大量使用杀虫剂防治害虫不仅带来了抗药性的威胁，而且对食品和环境造成了严重的污染。目前已经开发了产生针对昆虫基因的双链RNA（dsRNA）的转基因植物以保护免受昆虫害虫。在本研究中，我们使用该技术沉默棉铃虫精氨酸激酶（哈克）基因，编码磷酸转移酶，在细胞能量代谢中起着关键作用的无脊椎动物。通过农杆菌介导的转化产生哈克dsRNA的转基因拟南芥植物。当H.用转基因植物叶子喂养棉铃虫一龄幼虫3天，这显著高于对照组中记录的18%死亡率。此外，转基因植物的摄入显着延缓幼虫的生长，和哈克的转录水平也敲低了高达52%。取食生物测定进一步表明，抑制效率与转基因植物中产生的哈克dsRNA的完整性和浓度相关。这些结果有力地表明，H。在转基因拟南芥植株中，AK基因的表达水平得到了提高，表明AK基因的RNAi技术在害虫防治中具有潜在的应用价值。
Insect pests have caused noticeable economic losses in agriculture, and the heavy use of insecticide to control pests not only brings the threats of insecticide resistance but also causes the great pollution to foods and the environment. Transgenic plants producing double-stranded RNA (dsRNA) directed against insect genes have been is currently developed for protection against insect pests. In this study, we used this technology to silence the arginine kinase (AK) gene of Helicoverpa armigera (HaAK), encoding a phosphotransferase that plays a critical role in cellular energy metabolism in invertebrate. Transgenic Arabidopsis plants producing HaAK dsRNA were generated by Agrobacterium-mediated transformation. The maximal mortality rate of 55% was reached when H. armigera first-instar larvae were fed with transgenic plant leaves for 3 days, which was dramatically higher than the 18% mortality recorded in the control group. Moreover, the ingestion of transgenic plants significantly retarded larval growth, and the transcript levels of HaAK were also knocked down by up to 52%. The feeding bioassays further indicated that the inhibition efficiency was correlated with the integrity and concentration of the produced HaAK dsRNA in transgenic plants. These results strongly show that the resistance to H. armigera was improved in transgenic Arabidopsis plants, suggesting that the RNAi targeting of AK has the potential for the control of insect pests.
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影响因子：
11.4
作者：
Bouche, Nicolas;Lauressergues, Dominique;Vaucheret, Herve
通讯作者：
Vaucheret, Herve
影响因子：
46.9
作者：
Tabashnik, Bruce E.;Gassmann, Aaron J.;Carriere, Yves
通讯作者：
Carriere, Yves
影响因子：
3.7
作者：
Wuriyanghan H;Falk BW
通讯作者：
Falk BW
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
3.8
作者：
Ghanim, Murad;Kontsedalov, Svetlana;Czosnek, Henryk
通讯作者：
Czosnek, Henryk