Synergistic apoptosis-inducing effects on A375 human melanoma cells of natural borneol and curcumin.
Synergistic apoptosis-inducing effects on A375 human melanoma cells of natural borneol and curcumin.
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DOI：
10.1371/journal.pone.0101277
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wong YS
中科院分区：
文献类型：
--
作者：
Chen J;Li L;Su J;Li B;Chen T;Wong YS
本研究通过MTT法、流式细胞术和Western blotting探讨NB/Cur对人黑色素瘤A375细胞系生长和凋亡的协同作用。我们的结果表明，NB 与 Cur 有效协同，通过诱导细胞凋亡来增强其对 A375 人黑色素瘤细胞的抗增殖活性，亚 G1 细胞群、DNA 断裂、PARP 裂解和 caspase 激活的增加证明了这一点。 Western blotting进一步的机制研究表明，NB/Cur处理细胞后，磷酸化JNK表达水平上调，磷酸化ERK和Akt表达水平下调，导致A375细胞凋亡。此外，NB还增强Cur触发细胞内ROS过量产生和DNA损伤，上调磷酸化ATM、磷酸化Brca1和磷酸化p53的表达水平。结果表明 NB 和 Cur 在癌症治疗中的联合应用潜力。
This study was to investigate the synergistic effect of NB/Cur on growth and apoptosis in A375 human melanoma cell line by MTT assay, flow cytometry and Western blotting. Our results demonstrated that NB effectively synergized with Cur to enhance its antiproliferative activity on A375 human melanoma cells by induction of apoptosis, as evidenced by an increase in sub-G1 cell population, DNA fragmentation, PARP cleavage and caspase activation. Further mechanistic studies by Western blotting showed that after treatment of the cells with NB/Cur, up-regulation of the expression level of phosphorylated JNK and down-regulation of the expression level of phosphorylated ERK and Akt contributed to A375 cells apoptosis. Moreover, NB also potentiated Cur to trigger intracellular ROS overproduction and the DNA damage with up-regulation of the expression level of phosphorylated ATM, phosphorylated Brca1 and phosphorylated p53. The results indicate the combinational application potential of NB and Cur in treatments of cancers.
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