Assessing bioink shape fidelity to aid material development in 3D bioprinting.
Assessing bioink shape fidelity to aid material development in 3D bioprinting.
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DOI：
10.1088/1758-5090/aa90e2
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发表时间：
2017-11-30
期刊：
影响因子：
9
通讯作者：
Malda J
中科院分区：
文献类型：
--
作者：
Ribeiro A;Blokzijl MM;Levato R;Visser CW;Castilho M;Hennink WE;Vermonden T;Malda J
在基于挤出的生物打印期间，沉积的生物墨水长丝经受变形，例如悬垂长丝的塌陷，这损害了堆叠若干层生物墨水的能力，以及相邻长丝之间的融合，这损害了所需孔结构的分辨率和维持。当开发新的生物墨水时，在打印后评估其形状保真度的方法将有益于评估沉积的细丝的变形程度并估计最终打印的构造与设计的相似程度。然而，形状保真度已经预先通过印刷后的视觉检查来定性地评估，这妨碍了不同生物油墨的可印刷性的直接比较。在本技术说明中，我们提出了一种基于测试悬垂结构上的细丝塌陷和平行打印股的细丝融合的生物墨水形状保真度的定量评估。两个测试都应用于基于泊洛沙姆407和聚（乙二醇）共混物的水凝胶平台上，提供了具有不同屈服应力的水凝胶库。所提出的方法是一种评估生物墨水形状保真度的简单方法，适用于任何基于生物墨水的生物打印系统，并且能够基于打印丝的变形程度定量评估这方面的可打印性。此外，我们建立了一个简单的理论模型，将细丝塌陷与生物墨水屈服应力联系起来。两种形状保真度测试的结果都强调了屈服应力作为影响生物油墨的可印刷性的参数之一的作用。所提出的定量评价将允许在不同生物墨水平台之间进行可再现的比较。
During extrusion-based bioprinting, the deposited bioink filaments are subjected to deformations, such as collapse of overhanging filaments, which compromises the ability to stack several layers of bioink, and fusion between adjacent filaments, which compromises the resolution and maintenance of a desired pore structure. When developing new bioinks, approaches to assess their shape fidelity after printing would be beneficial to evaluate the degree of deformation of the deposited filament and to estimate how similar the final printed construct would be to the design. However, shape fidelity has been prevalently assessed qualitatively through visual inspection after printing, hampering the direct comparison of the printability of different bioinks. In this technical note, we propose a quantitative evaluation for shape fidelity of bioinks based on testing the filament collapse on overhanging structures and the filament fusion of parallel printed strands. Both tests were applied on a hydrogel platform based on poloxamer 407 and poly(ethylene glycol) blends, providing a library of hydrogels with different yield stresses. The presented approach is an easy way to assess bioink shape fidelity, applicable to any filament-based bioprinting system and able to quantitatively evaluate this aspect of printability, based on the degree of deformation of the printed filament. In addition, we built a simple theoretical model that relates filament collapse with bioink yield stress. The results of both shape fidelity tests underline the role of yield stress as one of the parameters influencing the printability of a bioink. The presented quantitative evaluation will allow for reproducible comparisons between different bioink platforms.
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影响因子：
9.7
作者：
Levato R;Webb WR;Otto IA;Mensinga A;Zhang Y;van Rijen M;van Weeren R;Khan IM;Malda J
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影响因子：
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影响因子：
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作者：
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通讯作者：
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