Postnatal growth retardation is associated with intestinal mucosa mitochondrial dysfunction and aberrant energy status in piglets.
Postnatal growth retardation is associated with intestinal mucosa mitochondrial dysfunction and aberrant energy status in piglets.
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仔猪出生后生长迟缓与肠粘膜线粒体功能障碍和能量状态异常有关
DOI：
10.1111/jcmm.15621
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发表时间：
2020-09
影响因子：
5.3
通讯作者：
Yin Y
中科院分区：
文献类型：
--
作者：
Qi M;Wang J;Tan B;Liao S;Long C;Yin Y
出生后生长迟缓（PGR）的个体容易发展为慢性疾病。小肠发育异常与生长性能受损有偶然联系。然而，确切的机制仍然未知。本研究以42日龄的PGR仔猪为模型，研究了仔猪小肠粘膜营养吸收和能量代谢的变化。结果显示，PGR仔猪血清中游离氨基酸和脂质代谢产物浓度较低，这与空肠和回肠粘膜中脂肪酸和氨基酸转运体相关mRNA表达下调一致。消化酶活性降低，线粒体肿胀。此外，还发现，低ATP产量，较高的AMP/ATP比值，恶化线粒体复合物III和ATP合成酶，并降低锰超氧化物歧化酶活性的PGR仔猪肠粘膜。此外，在PGR仔猪的肠粘膜中观察到参与能量代谢的基因表达改变，伴随SIRT 1、PGC-1α和PPARγ的蛋白丰度降低，以及AMPKα、mTOR、P70 S6 K和4 E-BP 1的磷酸化。总之，营养吸收能力下降，线粒体功能障碍，空肠和回肠粘膜的能量状态异常可能有助于PGR仔猪。
Individuals with postnatal growth retardation (PGR) are prone to developing chronic disease. Abnormal development in small intestine is casually implicated in impaired growth performance. However, the exact mechanism is still unknown. In this present study, PGR piglets (aged 42 days) were employed as a good model to analyse changes in nutrient absorption and energy metabolism in the intestinal mucosa. The results showed lower serum concentrations of free amino acids, and lipid metabolites in PGR piglets, which were in accordance with the down‐regulated mRNA expressions involved in fatty acid and amino acid transporters in the jejunal and ileal mucosa. The decreased activities of digestive enzymes and the marked swelling in mitochondria were also observed in the PGR piglets. In addition, it was found that lower ATP production, higher AMP/ATP ratio, deteriorated mitochondrial complex III and ATP synthase, and decreased manganese superoxide dismutase activity in the intestinal mucosa of PGR piglets. Furthermore, altered gene expression involved in energy metabolism, accompanied by decreases in the protein abundance of SIRT1, PGC‐1α and PPARγ, as well as phosphorylations of AMPKα, mTOR, P70S6K and 4E‐BP1 were observed in intestinal mucosa of PGR piglets. In conclusion, decreased capability of nutrient absorption, mitochondrial dysfunction, and aberrant energy status in the jejunal and ileal mucosa may contribute to PGR piglets.
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