Young developmental age cardiac extracellular matrix promotes the expansion of neonatal cardiomyocytes in vitro.
Young developmental age cardiac extracellular matrix promotes the expansion of neonatal cardiomyocytes in vitro.
复制标题
年轻的发育年龄心脏外基质促进了新生儿心肌细胞在体外的扩张。
DOI：
10.1016/j.actbio.2013.08.037
复制
发表时间：
2014-01
影响因子：
9.7
通讯作者：
Black, L. D., III
中科院分区：
文献类型：
--
作者：
Williams, C.;Quinn, K. P.;Georgakoudi, I.;Black, L. D., III
关键词：
心脏组织工程和再生医学策略的一个主要局限是出生后心肌细胞缺乏增殖能力。细胞外基质（ECM）在心脏发育过程中发生改变，研究表明它在调节心肌细胞增殖方面起着重要作用。在此，我们研究了胎儿、新生儿和成年心脏ECM对新生大鼠心室细胞体外扩增的影响。在24小时时，细胞在胎儿ECM上的总体附着率最低；然而，约80%的细胞是心肌细胞，而许多非心肌细胞附着在较年长的ECM和多聚赖氨酸对照物上。5天后，胎儿ECM上的心肌细胞数量仍然最多，增加了4倍。与其他基质相比，在5天时，胎儿ECM上有更多的心肌细胞对有丝分裂标记物磷酸化组蛋白H3呈阳性染色，这表明增殖可能是心肌细胞在年轻ECM上扩增的主要机制。对发育早期心脏ECM有益特性的进一步研究可能会推进旨在促进心脏再生的新型生物材料的设计。
A major limitation to cardiac tissue engineering and regenerative medicine strategies is the lack of proliferation of postnatal cardiomyocytes. The extracellular matrix (ECM) is altered during heart development and studies suggest it plays an important role in regulating myocyte proliferation. Here, we studied the effects of fetal, neonatal, and adult cardiac ECM on the expansion of neonatal rat ventricular cells in vitro. At 24 hr, overall cell attachment was lowest on fetal ECM; however ∼80% of the cells were cardiomyocytes while many non-myocytes attached to older ECM and poly-L-lysine controls. After 5 days, the cardiomyocyte population remained highest on fetal ECM, with a 4-fold increase in number. Significantly more cardiomyocytes stained positively for the mitotic marker phospho-histone H3 on fetal ECM compared to other substrates at 5 days, suggesting that proliferation may be a major mechanism of cardiomyocyte expansion on young ECM. Further study of the beneficial properties of early developmental aged cardiac ECM could advance the design of novel biomaterials aimed at promoting cardiac regeneration.
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影响因子：
5
作者：
Bowers SL;Banerjee I;Baudino TA
通讯作者：
Baudino TA
影响因子：
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影响因子：
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Leda, Masaki;Tsuchihashi, Takatoshi;Ivey, Kathryn N.;Ross, Robert S.;Hong, Ting-Ting;Shaw, Robin M.;Srivastava, Deepak
通讯作者：
Srivastava, Deepak
影响因子：
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作者：
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通讯作者：
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