The piggyBac-derived protein 5 (PGBD5) transposes both the closely and the distantly related piggyBac-like elements Tcr-pble and Ifp2.
The piggyBac-derived protein 5 (PGBD5) transposes both the closely and the distantly related piggyBac-like elements Tcr-pble and Ifp2.
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DOI：
10.1016/j.jmb.2021.166839
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发表时间：
2021-04-02
影响因子：
5.6
通讯作者：
Bigot Y
中科院分区：
文献类型：
--
作者：
Helou L;Beauclair L;Dardente H;Piégu B;Tsakou-Ngouafo L;Lecomte T;Kentsis A;Pontarotti P;Bigot Y
脊椎动物piggyBac衍生的转座酶5（PGBD 5）编码驯化的转座酶，其是有活性的并且能够转座其远亲的piggyBac样元件（pble）Ifp 2。这就提出了一个问题，即PGBD 5是否能更有效地动员一个与遗传学密切相关的pble元件。我们的目的是确定pble最密切相关的pgbd 5基因。我们更新了脊椎动物pgbd基因的景观，以开发有效的过滤器，并确定与这些基因中的每一个最密切相关的pble。我们发现Tcr-pble在遗传学上是与pgbd 5基因最接近的pble。此外，我们评估了两种鼠和人PGBD 5亚型Mm 523和Hs 524转座Tcr-pble和Ifp 2元件的能力。我们发现，这两个pbles可以转座Mm 523具有类似的效率。然而，这两个pbles的整合发生通过适当的转座和不适当的PGBD 5依赖性重组。这表明PGBD 5结合两个pbles的能力可能不是基于元件末端的一级序列，而是可能涉及识别内部DNA基序，可能与回文重复有关。与此假设一致，我们确定了内部回文重复序列的24个pble序列，显示不同的序列的末端附近。
The vertebrate piggyBac derived transposase 5 (PGBD5) encodes a domesticated transposase, which is active and able to transpose its distantly related piggyBac-like element (pble), Ifp2. This raised the question whether PGBD5 would be more effective at mobilizing a phylogenetically closely related pble element. We aimed to identify the pble most closely related to the pgbd5 gene. We updated the landscape of vertebrate pgbd genes to develop efficient filters and identify the most closely related pble to each of these genes. We found that Tcr-pble is phylogenetically the closest pble to the pgbd5 gene. Furthermore, we evaluated the capacity of two murine and human PGBD5 isoforms, Mm523 and Hs524, to transpose both Tcr-pble and Ifp2 elements. We found that both pbles could be transposed by Mm523 with similar efficiency. However, integrations of both pbles occurred through both proper transposition and improper PGBD5-dependent recombination. This suggested that the ability of PGBD5 to bind both pbles may not be based on the primary sequence of element ends, but may involve recognition of inner DNA motifs, possibly related to palindromic repeats. In agreement with this hypothesis, we identified internal palindromic repeats near the end of 24 pble sequences, which display distinct sequences.
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影响因子：
9.5
作者：
Katoh K;Rozewicki J;Yamada KD
通讯作者：
Yamada KD
影响因子：
3.7
作者：
Bire S;Ley D;Casteret S;Mermod N;Bigot Y;Rouleux-Bonnin F
通讯作者：
Rouleux-Bonnin F
发现具有任意插入和删除的序列基序。
影响因子：
4.3
作者：
Frith, Martin C.;Saunders, Neil F. W.;Kobe, Bostjan;Bailey, Timothy L.
通讯作者：
Bailey, Timothy L.
影响因子：
4.9
作者：
Bao W;Kojima KK;Kohany O
通讯作者：
Kohany O
影响因子：
4
作者：
Joly-Lopez, Zoe;Bureau, Thomas E.
通讯作者：
Bureau, Thomas E.