Increased microRNA-30a levels in bronchoalveolar lavage fluid as a diagnostic biomarker for lung cancer.
Increased microRNA-30a levels in bronchoalveolar lavage fluid as a diagnostic biomarker for lung cancer.
复制标题
支气管肺泡灌洗液中 microRNA-30a 水平升高作为肺癌诊断生物标志物
作者：
Zhu WJ;Chen BJ;Zhu YY;Sun L;Zhang YC;Liu H;Luo FM
背景肺癌患者血浆中microRNA-30 a（miRNA-30 a）水平升高。在此，我们评估了肺癌患者支气管肺泡灌洗液（BALF）中miRNA-30 a水平作为肺癌诊断的潜在生物标志物。方法采用实时荧光定量逆转录-聚合酶链反应（RT-PCR）技术检测174例肺癌患者BALF中miRNA-30 a的表达，并与对照组进行比较。并通过受试者工作特征曲线（ROC）分析评价其诊断价值。结果肺癌患者BALF中miRNA-30 a的相对表达量明显高于对照组（分别为0.74 ± 0.55和0.07 ± 0.48，p < 0.001）以及I-IIA期肺癌患者比IIB-IV期肺癌患者（分别为0.98 ± 0.64和0.66 ± 0.54，p < 0.05）。此外，miRNA-30 a将良性肺病与肺癌区分开来，ROC曲线下面积（AUC）为0.822。ROC分析还显示基于Youden指数的I-IIA期腺癌最佳截止点的AUC为0.875。因此，BALF中增加的miRNA-30 a水平可能是非小细胞肺癌诊断的有用生物标志物。
Background MicroRNA-30a (miRNA-30a) levels have been shown to increase in the plasma of lung cancer patients. Herein, we evaluated the miRNA-30a levels in the bronchoalveolar lavage fluid (BALF) of lung cancer patients as a potential biomarker for lung cancer diagnosis. Methods BALF miRNA-30a expression of 174 subjects was quantified using quantitative real-time reverse transcription-polymerase chain reaction and compared between lung cancer patients and control patients with benign lung diseases. Moreover, its diagnostic value was evaluated by performing receiver operating characteristic (ROC) curve analysis. Results The relative BALF miRNA-30a expression was significantly higher in the lung cancer patients than in the controls (0.74 ± 0.55 versus 0.07 ± 0.48, respectively, p < 0.001) as well as in lung cancer patients with stage I–IIA disease than in those with stage IIB–IV disease (0.98 ± 0.64 versus 0.66 ± 0.54, respectively, p < 0.05). Additionally, miRNA-30a distinguished benign lung diseases from lung cancers, with an area under the ROC curve (AUC) of 0.822. ROC analysis also revealed an AUC of 0.875 for the Youden index-based optimal cut-off points for stage I–IIA adenocarcinoma. Thus, increased miRNA-30a levels in BALF may be a useful biomarker for non-small-cell lung cancer diagnosis.
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