Changes in gene expression profiles as they relate to the adult plant leaf rust resistance in the wheat cv. Toropi.
Changes in gene expression profiles as they relate to the adult plant leaf rust resistance in the wheat cv. Toropi.
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基因表达曲线的变化与小麦CV中的成年植物叶锈病有关。 Toropi。
DOI：
10.1016/j.pmpp.2014.12.004
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发表时间：
2015-01
影响因子：
2.7
通讯作者：
Boyd, Lesley A.
中科院分区：
文献类型：
--
作者：
Casassola, Alice;Brammer, Sandra P.;Chaves, Marcia S.;Martinelli, Jose A.;Stefanato, Francesca;Boyd, Lesley A.
叶锈病由叶病原体小麦柄锈菌（Puccinia triticina）引起，是巴西南部地区小麦的主要病害，并且总是影响生产，造成高产量损失。巴西小麦栽培品种Toropi已被证明对叶锈病具有持久的成株抗性（APR），其独特地显示出前吸器抗性表型。在这项研究中，我们的目的是了解P. triticina和前吸器APR在Toropi之间的相互作用，通过定量评估已知的小麦感染和防御相关的基因的时间转录谱。15个选定基因的表达谱随着时间的推移而变化，分为六个表达谱组。表达谱表明响应于病原体发展的经典防御途径的诱导，但也可能为了病原体的利益而改变Toropi的细胞状态。经典的防御基因，包括过氧化物酶，β-1，3-葡聚糖酶和内切几丁质酶的表达早期（前吸器）和晚期（后吸器）超过72小时的感染时间过程中，而诱导转录的其他感染相关基因的防御中的潜在作用，虽然变量保持了。这些基因直接或间接地参与植物木质化、氧化胁迫、能量供应、水分和脂类运输、细胞周期调控等过程。小麦抗叶锈相关基因转录的早期诱导支持了Toropi的前吸器抗性表型，为小麦抗叶锈育种提供了有价值的基因来源。小麦柄锈菌侵染相关基因分为6个表达谱。Toropi的前吸器APR与早期防御基因的转录有关。诱导经典的防御途径，在响应病原体的发展。与真菌修饰植物细胞状态相关的基因诱导。
Leaf rust, caused by the foliar pathogen Puccinia triticina is a major disease of wheat in the southern region of Brazil and invariably impacts on production, being responsible for high yield losses. The Brazilian wheat cultivar Toropi has proven, durable adult plant resistance (APR) to leaf rust, which uniquely shows a pre-haustorial resistance phenotype. In this study we aimed to understand the interaction between P. triticina and the pre-haustorial APR in Toropi by quantitatively evaluating the temporal transcription profiles of selected genes known to be related to infection and defense in wheat. The expression profiles of 15 selected genes varied over time, grouping into six expression profile groups. The expression profiles indicated the induction of classical defence pathways in response to pathogen development, but also the potential modification of Toropi's cellular status for the benefit of the pathogen. Classical defence genes, including peroxidases, β-1,3-glucanases and an endochitinase were expressed both early (pre-haustorial) and late (post-haustorial) over the 72 h infection time course, while induction of transcription of other infection-related genes with a potential role in defence, although variable was maintained through-out. These genes directly or indirectly had a role in plant lignification, oxidative stress, the regulation of energy supply, water and lipid transport, and cell cycle regulation. The early induction of transcription of defence-related genes supports the pre-haustorial resistance phenotype in Toropi, providing a valuable source of genes controlling leaf rust resistance for wheat breeding. Puccinia triticina infection-related genes grouped into six expression profiles. Pre-haustorial APR in Toropi was associated with early defence gene transcription. Induction of classical defence pathways seen in response to pathogen development. Gene induction associated with fungal-modification of the plant's cellular status.
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影响因子：
4.5
作者：
Barcellos, AL;Roelfs, AP;de Moraes-Fernandes, MIB
通讯作者：
de Moraes-Fernandes, MIB
影响因子：
4.1
作者：
Dynowski, Marek;Schaaf, Gabriel;Ludewig, Uwe
通讯作者：
Ludewig, Uwe
DOI：
10.1016/j.bbaexp.2005.06.010
发表时间：
2005-08-15
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Boutrot, F;Guirao, A;Gautier, MF
通讯作者：
Gautier, MF
影响因子：
64.8
作者：
Collins, NC;Thordal-Christensen, H;Schulze-Lefert, P
通讯作者：
Schulze-Lefert, P
影响因子：
2.7
作者：
Kolmer, J. A.;Hanzalova, A.;Morgounov, A.
通讯作者：
Morgounov, A.