Absence of hematopoiesis from transplanted olfactory bulb neural stem cells
Absence of hematopoiesis from transplanted olfactory bulb neural stem cells
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移植的嗅球神经干细胞缺乏造血功能
DOI：
10.1111/j.1460-9568.2004.03140.x
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发表时间：
2004
影响因子：
3.4
通讯作者：
C. Vicario
中科院分区：
文献类型：
--
作者：
M. J. Yusta;Manuel A. González;N. Pavón;A. B. Martín;R. de la Fuente;J. García;F. de Pablo;R. Moratalla;A. Bernad;C. Vicario
神经干细胞产生神经元和神经胶质细胞已经从胚胎和成人中枢神经系统分离。然而，它们能够分化成非神经谱系细胞（如造血谱系）的程度尚不清楚。我们先前报道了从小鼠嗅球神经上皮分离干细胞。在本研究中，我们分析了嗅球干细胞（OBSC）是否可以产生具有造血功能的细胞。从表达增强型绿色荧光蛋白（EGFP）的转基因小鼠的嗅球制备细胞。在培养中，转基因细胞以与野生型细胞相同的动力学增殖。去除促分裂原后，两种细胞类型产生了相似数量的神经元、星形胶质细胞和少突胶质细胞，表明EGFP过表达不会改变OBSC的增殖和分化模式。当将这些细胞注射到受辐射小鼠的尾静脉中时，在其外周血、脾或骨髓中不能回收来自OBSC的造血细胞。相比之下，当OBSC被移植到成人大脑中时，在纹状体和胼胝体中发现了EGFP阳性细胞;分化的细胞表达神经元和星形胶质细胞的抗原标记物。这些结果表明，胚胎嗅球干细胞没有被赋予产生造血的潜力。
Neural stem cells giving rise to neurons and glia cells have been isolated from the embryonic and adult central nervous system. The extent to which they are able to differentiate into cells of non‐neural lineages, such as the hematopoietic lineage, is nonetheless unclear. We previously reported the isolation of stem cells from the mouse olfactory bulb neuroepithelium. In the present study, we analysed whether olfactory bulb stem cells (OBSC) can generate cells with hematopoietic features. Cells were prepared from the olfactory bulbs of transgenic mice expressing enhanced green fluorescent protein (EGFP). In culture, transgenic cells proliferated with the same kinetics as wild‐type cells. Following mitogen removal, both cell types gave rise to similar numbers of neurons, astrocytes and oligodendrocytes, indicating that EGFP overexpression does not alter OBSC proliferation and differentiation patterns. When these cells were injected into the tail vein of irradiated mice, no hematopoietic cells derived from the OBSC could be recovered in their peripheral blood, spleen or bone marrow. By contrast, when OBSC were transplanted into the adult brain, EGFP‐positive cells were found in the striatum and corpus callosum; differentiated cells expressed antigenic markers of neurons and astrocytes. These results suggest that embryonic olfactory bulb stem cells are not endowed with the potential to produce hematopoiesis.
影响因子：
20.3
作者：
Shih,CC;Weng,Y;Mamelak,A;LeBon,T;Hu,MC;Forman,SJ
通讯作者：
Forman,SJ
影响因子：
56.9
作者：
Brazelton, TR;Rossi, FMV;Blau, HM
通讯作者：
Blau, HM
影响因子：
56.9
作者：
Mezey, É;Chandross, KJ;McKercher, SR
通讯作者：
McKercher, SR