Invertase Suc2-mediated inulin catabolism is regulated at the transcript level in Saccharomyces cerevisiae.
Invertase Suc2-mediated inulin catabolism is regulated at the transcript level in Saccharomyces cerevisiae.
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转化酶 Suc2 介导的菊粉分解代谢在酿酒酵母的转录水平上受到调节
DOI：
10.1186/s12934-015-0243-3
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发表时间：
2015-04-17
影响因子：
6.4
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Yang F;Liu ZC;Wang X;Li LL;Yang L;Tang WZ;Yu ZM;Li X
背景：蔗糖酶Suc2是近年来发现的一种重要的菊糖催化水解酶，但不同的酿酒酵母Suc2降解菊糖的活性差异很大。酿酒酵母菌株。导致这种变异的分子机制仍然不清楚。本研究的目的是研究Suc2活性在S.结果：通过将不同的SUC2基因或其相应的启动子导入酿酒酵母，研究了SUC2表达水平对菊粉水解的影响。酿酒酵母菌株BY4741仅能弱降解菊粉。菊粉酶和转化酶活性均随SUC2表达水平的提高而提高。启动子序列的变异对SUC2基因的转录水平有明显的影响。结论：Suc2介导的菊糖催化作用在S.啤酒。本研究为进一步利用菊粉生产乙醇提供了理论依据。
Background:Invertase Suc2 was recently identified as a key hydrolase for inulin catabolism in Saccharomyces cerevisiae, whereas the Suc2 activity degrading inulin varies greatly in different S. cerevisiae strains. The molecular mechanism causing such variation remained obscure. The aim of this study is to investigate how Suc2 activity is regulated in S. cerevisiae.Results:The effect of SUC2 expression level on inulin hydrolysis was investigated by introducing different SUC2 genes or their corresponding promoters in S. cerevisiae strain BY4741 that can only weakly catabolize inulin. Both inulinase and invertase activities were increased with the rising SUC2 expression level. Variation in the promoter sequence has an obvious effect on the transcript level of the SUC2 gene. It was also found that the high expression level of SUC2 was beneficial to inulin degradation and ethanol yield.Conclusions:Suc2-mediated inulin catabolism is regulated at transcript level in S. cerevisiae. Our work should be valuable for engineering advanced yeast strains in application of inulin for ethanol production.
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影响因子：
6
作者：
Lane, Melanie M.;Morrissey, John P.
通讯作者：
Morrissey, John P.
耐热马克斯克鲁维酵母和酿酒酵母菌株代表了通过综合生物加工从菊芋生产生物乙醇的潜力
影响因子：
5
作者：
Hu, Nan;Yuan, Bo;Li, Fu-Li
通讯作者：
Li, Fu-Li
影响因子：
14.9
作者：
TAUSSIG, R;CARLSON, M
通讯作者：
CARLSON, M
影响因子：
2.6
作者：
Schorr-Galindo, S;Ghommidh, C;Guiraud, JP
通讯作者：
Guiraud, JP
DOI：
10.1002/(sici)1097-0134(19981115)33:3
发表时间：
1998-11-15
期刊：
PROTEINS-STRUCTURE FUNCTION AND GENETICS
影响因子：
--
作者：
Pons, T;Olmea, O;Valencia, A
通讯作者：
Valencia, A