Mycobacteria isolated from angkor monument sandstones grow chemolithoautotrophically by oxidizing elemental sulfur.
Mycobacteria isolated from angkor monument sandstones grow chemolithoautotrophically by oxidizing elemental sulfur.
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DOI：
10.3389/fmicb.2011.00104
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发表时间：
2011
影响因子：
5.2
通讯作者：
Katayama Y
中科院分区：
文献类型：
--
作者：
Kusumi A;Li XS;Katayama Y
为了鉴定柬埔寨吴哥古迹变质砂岩中的硫酸盐产生微生物，分枝杆菌属的菌株。是从大多数可能的数字阳性培养中分离出来的。分离到的5株细菌均能利用元素硫(S0)进行化学自养生长，也能利用有机物进行化学有机异养生长。系统发育和表型分析结果表明，这5个菌株都是分枝杆菌属的快速生长菌，与化妆品分枝杆菌和苍白分枝杆菌最为相似。对代表菌株THI503的自养生长进行了进一步研究。当菌株THI503在无机盐介质中生长时，将S0氧化成硫代硫酸盐和硫酸盐；氧化伴随着介质的pH从4.7下降到3.6。硫磺氧化和能量代谢之间的联系被ATP的增加所证实。DAPI染色细胞的荧光显微镜显示，菌株THI503在硫磺颗粒表面黏附和增殖。兼性化学自养细胞的灵活代谢能力使它们能够在营养有限的砂岩环境中生存。
To characterize sulfate-producing microorganisms from the deteriorated sandstones of Angkor monuments in Cambodia, strains of Mycobacterium spp. were isolated from most probable number-positive cultures. All five strains isolated were able to use both elemental sulfur (S0) for chemolithoautotrophic growth and organic substances for chemoorganoheterotrophic growth. Results of phylogenetic and phenotypic analyses indicated that all five isolates were rapid growers of the genus Mycobacterium and were most similar to Mycobacterium cosmeticum and Mycobacterium pallens. Chemolithoautotrophic growth was further examined in the representative strain THI503. When grown in mineral salts medium, strain THI503 oxidized S0 to thiosulfate and sulfate; oxidation was accompanied by a decrease in the pH of the medium from 4.7 to 3.6. The link between sulfur oxidation and energy metabolism was confirmed by an increase in ATP. Fluorescence microscopy of DAPI-stained cells revealed that strain THI503 adheres to and proliferates on the surface of sulfur particles. The flexible metabolic ability of facultative chemolithoautotrophs enables their survival in nutrient-limited sandstone environments.
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影响因子：
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