Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae.
Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae.
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DOI：
10.1128/iai.00565-21
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发表时间：
2022-04-21
影响因子：
3.1
通讯作者：
中科院分区：
文献类型：
--
作者：
LAV是在致病性嗜血杆菌和奈瑟氏菌属物种中发现的自转运蛋白。LAV在不可分型的流感嗜血杆菌（NTHi）中是相位可变的：该基因通过改变位于基因座的GCAA（n）简单DNA序列重复区的长度可逆地开关ON-OFF。在NTHi的携带和侵入性集合中检查Iav的表达状态，其中Iav主要不表达（OFF）。表型研究显示lav表达（ON）导致对人肺细胞的粘附增加和更致密的生物膜形成。对嗜血杆菌属物种基因组序列的调查显示，lav存在于160%的NTHi菌株中，但在大多数可分型的嗜血杆菌中不存在lav。流感病毒株。序列分析揭示了嗜血杆菌属中存在的Lav乘客结构域的总共五种不同变体，这五种变体显示出不同的谱系分布。确定LAV在NTHi中的作用将有助于理解这种蛋白在不同病理过程中的作用。
Lav is an autotransporter protein found in pathogenic Haemophilus and Neisseria species. Lav in nontypeable Haemophilus influenzae (NTHi) is phase-variable: the gene reversibly switches ON-OFF via changes in length of a locus-located GCAA(n) simple DNA sequence repeat tract. The expression status of lav was examined in carriage and invasive collections of NTHi, where it was predominantly not expressed (OFF). Phenotypic study showed lav expression (ON) results in increased adherence to human lung cells and denser biofilm formation. A survey of Haemophilus species genome sequences showed lav is present in ∼60% of NTHi strains, but lav is not present in most typeable H. influenzae strains. Sequence analysis revealed a total of five distinct variants of the Lav passenger domain present in Haemophilus spp., with these five variants showing a distinct lineage distribution. Determining the role of Lav in NTHi will help understand the role of this protein during distinct pathologies.
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影响因子：
2.1
作者：
Pereira RFC;Theizen TH;Machado D;Guarnieri JPO;Gomide GP;Hollanda LM;Lancellotti M
通讯作者：
Lancellotti M
影响因子：
3.1
作者：
Palmu, Arto A.;Ware, Robert S.;Grimwood, Keith
通讯作者：
Grimwood, Keith
影响因子：
2.9
作者：
Lambert, Stephen Bernard;Ware, Robert S.;Grimwood, Keith
通讯作者：
Grimwood, Keith
影响因子：
8.8
作者：
Harrison，Alistair;Hardison，Rachael L.;Mason，Kevin M.
通讯作者：
Mason，Kevin M.
影响因子：
5.2
作者：
Atkinson, Christopher T.;Kunde, Dale A.;Tristram, Stephen G.
通讯作者：
Tristram, Stephen G.