Emergence of carbapenem-resistant Klebsiella pneumoniae harbouring bla (OXA-48)-like genes in China.
Emergence of carbapenem-resistant Klebsiella pneumoniae harbouring bla (OXA-48)-like genes in China.
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中国中出现耐碳青霉烯类肺炎克雷伯菌携带blA(oxA-48)样基因。
DOI：
10.1099/jmm.0.001306
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发表时间：
2021-03
影响因子：
3
通讯作者：
Yu Y
中科院分区：
文献类型：
--
作者：
Chen Y;Fang L;Yang Y;Yan R;Fu Y;Shen P;Zhao D;Chen Y;Hua X;Jiang Y;Moran RA;van Schaik W;Yu Y
肺炎克雷伯菌 携带OXA-48样碳青霉烯酶的菌株在地球仪中越来越普遍。因此，迫切需要更好地了解支持bla OXA-48样碳青霉烯酶传播的机制。为此，四种厄他培南耐药 K.肺炎 产生OXA-48样碳青霉烯酶的分离物分离自两名患者。基因组测序显示，一个序列类型（ST）17分离株携带bla OXA-181，而来自同一患者的三个分离株（两个ST 76和一个ST 15）携带bla OXA-232。含有50514 bp bla OXA-181的质粒pOXA-181_YML 0508为X3型，接合频率为 大肠杆菌 每个供体1.94×10−4个接合子。bla OXA-232基因位于6141 bp ColKP 3型质粒pOXA-232_WSD上，该质粒在ST 76和ST 15中相同 K.肺炎 分离株这种质粒可以从 K.肺炎 到 E.杆菌 在低频率下，每个供体有8.13×10 - 6个接合子。比较分析表明，X3质粒通过IS 3000介导的ColKP 3型质粒的共整合获得bla OXA-48-like基因。我们的研究强调了质粒整合和重排如何有助于bla OXA-48样基因的传播，这为临床预防大肠杆菌的传播提供了重要线索。 K.肺炎 携带bla OXA-48样碳青霉烯酶的菌株。
Klebsiella pneumoniae strains carrying OXA-48-like carbapenemases are increasingly prevalent across the globe. There is thus an urgent need to better understand the mechanisms that underpin the dissemination of bla OXA-48-like carbapenemases. To this end, four ertapenem-resistant K. pneumoniae isolates producing OXA-48-like carbapenemases were isolated from two patients. Genome sequencing revealed that one sequence type (ST) 17 isolate carried bla OXA-181, whilst three isolates from a single patient, two ST76 and one ST15, carried bla OXA-232. The 50514 bp bla OXA-181-harbouring plasmid, pOXA-181_YML0508, was X3-type with a conjugation frequency to Escherichia coli of 1.94×10−4 transconjugants per donor. The bla OXA-232 gene was located on a 6141 bp ColKP3-type plasmid, pOXA-232_WSD, that was identical in the ST76 and ST15 K. pneumoniae isolates. This plasmid could be transferred from K. pneumoniae to E. coli at low frequency, 8.13×10−6 transconjugants per donor. Comparative analysis revealed that the X3 plasmid acquired the bla OXA-48-like gene via IS3000-mediated co-integration of the ColKP3-type plasmid. Our study highlights how plasmid integration and rearrangements can contribute to the spread of bla OXA-48-like genes, which provides important clues for clinical prevention of the dissemination of K. pneumoniae strains carrying bla OXA-48-like carbapenemases.
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