Ca2+ release by inositol-trisphosphorothioate in isolated triads of rabbit skeletal muscle.
Ca2+ release by inositol-trisphosphorothioate in isolated triads of rabbit skeletal muscle.
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肌醇三硫代磷酸酯在分离的兔骨骼肌三联体中释放 Ca2+。
DOI：
10.1016/s0006-3495(90)82642-4
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发表时间：
1990
影响因子：
3.4
通讯作者：
Coronado,R
中科院分区：
文献类型：
--
作者：
Valdivia,C;Valdivia,HH;Potter,BV;Coronado,R
Cooke，A. M.，R.吉格和B。V. L. Potter，（1987 b. Jour. Chem. Soc. Chem. Commun.（1525-1526年）在从兔骨骼肌中纯化的三联体中检测。使用Ca 2+电极摄取-释放测定来确定分离的三联体中的IPS 3-可释放的Ca 2+池的大小和灵敏度。通过ImM MgATP（pCa 5.8，pH 7.5）启动摄取，当游离Ca 2+降低至pCa约7时启动释放。我们发现，5-25 μ M的肌醇1，4，5-三磷酸（IP 3），和单独的IPS 3，始终释放5-20%的钙池积极加载到三元组。单通道记录用于确定在相同的肌浆Ca 2+和IPS 3浓度下，IPS 3是否影响ryanodine受体Ca 2+释放通道。兰尼碱受体Ca 2+释放通道的开放概率进行了监测，在三合会融合到双层在长时间（200秒）的情况下，并添加30 μ M的IPS 3到同一个通道。在肌浆pCa约为7时，IPS 3在不存在MgATP的情况下没有作用（对照中Po = 0.0094 +/- 0.001，IPS 3后Po = 0.01 +/- 0.006），并且在存在ImM MgATP的情况下略微增加活性（对照中Po = 0.024 +/- 0.03，IPS 3后Po = 0.05 +/- 0.03）。在较高的肌浆Ca 2+水平下观察到同样小的影响。IPS 3或IP 3的通道激活的开始是缓慢的，在时间尺度上为20-60秒。我们认为，在孤立的三胞胎兔骨骼肌，IP 3诱导释放的存储Ca 2+可能不是介导的开放的Ca 2+释放通道。
The effectiveness of the nonmetabolizable second messenger analogue DL-myo-inositol 1,4,5-trisphosphorothioate (IPS3) described by Cooke, A. M., R. Gigg, and B. V. L. Potter, (1987b. Jour. Chem. Soc. Chem. Commun. 1525–1526.) was examined in triads purified from rabbit skeletal muscle. A Ca2+ electrode uptake-release assay was used to determine the size and sensitivity of the IPS3-releasable pool of Ca2+ in isolated triads. Uptake was initiated by 1 mM MgATP, pCa 5.8, pH 7.5 Release was initiated when the free Ca2+ had lowered to pCa approximately 7. We found that 5–25 microM myo-inositol 1,4,5-trisphosphate (IP3), and separately IPS3, consistently released 5–20% of the Ca2+ pool actively loaded into triads. Single channel recording was used to determine if ryanodine receptor Ca2+ release channels were affected by IPS3 at the same myoplasmic Ca2+ and IPS3 concentrations. Open probability of ryanodine receptor Ca2+ release channels was monitored in triads fused to bilayers over long periods (200 s) in the absence and following addition of 30 microM IPS3 to the same channel. At myoplasmic pCa approximately 7, IPS3 had no effect in the absence of MgATP (Po = 0.0094 +/- 0.001 in control and Po = 0.01 +/- 0.006 after IPS3) and slightly increased activity in the presence of 1 mM MgATP (Po = 0.024 +/- 0.03 in control and Po = 0.05 +/- 0.03 after IPS3). Equally small effects were observed at higher myoplasmic Ca2+. The onset of channel activation by IPS3 or IP3 was slow, on the time scale 20–60 s. We suggest that in isolated triads of rabbit skeletal muscle, IP3-induced release of stored Ca2+ is probably not mediated by the opening of Ca2+ release channels.
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分离的肌浆网中的钙快速释放是通过附着的横向管状系统触发的。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ikemoto,N;Antoniu,B;Kim,DH
通讯作者：
Kim,DH
肌醇 1,4,5-三磷酸不能有效地从骨骼肌浆网微粒体中释放钙。
DOI：
10.1016/0006-291x(85)91048-4
发表时间：
1985
影响因子：
3.1
作者：
N. Scherer;J. Ferguson
通讯作者：
J. Ferguson
肌醇(1,4,5)三磷酸硫代酯与大鼠小脑中的特定[3H]肌醇(1,4,5)三磷酸位点结合，并且对5-磷酸酶具有抗性。
影响因子：
5
作者：
A. L. Willcocks;B. Potter;A. Cooke;S. Nahorski
通讯作者：
S. Nahorski
影响因子：
64.8
作者：
H. Takeshima;S. Nishimura;Takeshi Matsumoto;Hiroyuki Ishida;K. Kangawa;N. Minamino;H. Matsuo;M. Ueda;M. Hanaoka;T. Hirose;S. Numa
通讯作者：
H. Takeshima;S. Nishimura;Takeshi Matsumoto;Hiroyuki Ishida;K. Kangawa;N. Minamino;H. Matsuo;M. Ueda;M. Hanaoka;T. Hirose;S. Numa
肌醇硫代磷酸酯，肌醇磷酸酯的磷酸酶抗性类似物。
DOI：
10.1042/bj2460771
发表时间：
1987
期刊：
The Biochemical journal
影响因子：
--
作者：
Michael R Hamblin;J. S. Flora;B. Potter
通讯作者：
B. Potter