White-tailed deer (Odocoileus virginianus) may serve as a wildlife reservoir for nearly extinct SARS-CoV-2 variants of concern.
White-tailed deer (Odocoileus virginianus) may serve as a wildlife reservoir for nearly extinct SARS-CoV-2 variants of concern.
复制标题
DOI：
10.1073/pnas.2215067120
复制
发表时间：
2023-02-07
影响因子：
11.1
通讯作者：
Diel, Diego G.
中科院分区：
文献类型：
--
作者：
Caserta, Leonardo C.;Martins, Mathias;Butt, Salman L.;Hollingshead, Nicholas A.;Covaleda, Lina M.;Ahmed, Sohel;Everts, Mia R. R.;Schuler, Krysten L.;Diel, Diego G.
这项全面的横断面研究表明，WTD在整个纽约州广泛感染SARS-CoV-2。我们显示了三种主要的SARS-CoV-2变异株(VOCs；Alpha、Delta和Gamma)在该物种中的共同循环，这是在上次在人类身上检测到它们之后很久的事情。有趣的是，从WTD中提取的病毒序列与从人类中提取的SARS-CoV-2序列高度不同，这表明该病毒在WTD中迅速适应。这些突变对病毒在WTD之间以及从WTD到人类的传播性的影响仍有待确定。总之，我们的发现表明，WTD--北美最丰富的大型哺乳动物--可能是不再在人类群体中传播的SARS-CoV-2变异毒株的储存库。严重急性呼吸综合征冠状病毒2(SARS-CoV-2)从人到白尾鹿(WTD)的溢出及其从鹿到鹿的传播能力引发了人们对WTD在病毒流行病学和生态学中所起作用的担忧。在这里，我们提出了一项全面的横断面研究，评估了纽约州WTD地区SARS-CoV-2的流行率、遗传多样性和进化。用SARS-CoV-2实时定量RT-PCR方法检测了2021年狩猎季节(2021年9月至12月)和2021年9月至12月狩猎季节(2021年9月至12月)采集的5462个自由放牧猎人采集的WTD咽后淋巴结样本。SARS-CoV-2RNA从第一季的17份样本(0.6%)和第二季的583份样本(21.1%)中检出。对164份样本的SARS-CoV-2基因组序列分析表明，WTD存在多个SARS-CoV-2谱系和三个主要的关注变种(VOC)(Alpha、Gamma和Delta)的共循环。我们的分析表明，阿尔法和三角洲血统发生了多次(人对鹿)溢出事件，随后发生了鹿对鹿的传播和适应病毒。在纽约人类广泛传播后很久，在WTD中检测到Alpha和Gamma变体，表明WTD可能是不再在人类中传播的VOCs的野生动物储藏库。因此，有必要实施持续的监测计划，以监测WTD的SARS-CoV-2动态，并迫切需要采取措施，将病毒在人与动物之间的传播降至最低。
This comprehensive cross-sectional study demonstrates widespread infection of WTD with SARS-CoV-2 across the State of New York. We showed cocirculation of three major SARS-CoV-2 variants of concern (VOCs; Alpha, Delta, and Gamma) in this species, long after their last detection in humans. Interestingly, the viral sequences recovered from WTD were highly divergent from SARS-CoV-2 sequences recovered from humans, suggesting rapid adaptation of the virus in WTD. The impact of these mutations on the transmissibility of the virus between WTD and from WTD to humans remains to be determined. Together, our findings indicate that WTD—the most abundant large mammal in North America—may serve as a reservoir for variant SARS-CoV-2 strains that no longer circulate in the human population. The spillover of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from humans to white-tailed deer (WTD) and its ability to transmit from deer to deer raised concerns about the role of WTD in the epidemiology and ecology of the virus. Here, we present a comprehensive cross-sectional study assessing the prevalence, genetic diversity, and evolution of SARS-CoV-2 in WTD in the State of New York (NY). A total of 5,462 retropharyngeal lymph node samples collected from free-ranging hunter-harvested WTD during the hunting seasons of 2020 (Season 1, September to December 2020, n = 2,700) and 2021 (Season 2, September to December 2021, n = 2,762) were tested by SARS-CoV-2 real-time RT–PCR (rRT-PCR). SARS-CoV-2 RNA was detected in 17 samples (0.6%) from Season 1 and in 583 samples (21.1%) from Season 2. Hotspots of infection were identified in multiple confined geographic areas of NY. Sequence analysis of SARS-CoV-2 genomes from 164 samples demonstrated the presence of multiple SARS-CoV-2 lineages and the cocirculation of three major variants of concern (VOCs) (Alpha, Gamma, and Delta) in WTD. Our analysis suggests the occurrence of multiple spillover events (human to deer) of the Alpha and Delta lineages with subsequent deer-to-deer transmission and adaptation of the viruses. Detection of Alpha and Gamma variants in WTD long after their broad circulation in humans in NY suggests that WTD may serve as a wildlife reservoir for VOCs no longer circulating in humans. Thus, implementation of continuous surveillance programs to monitor SARS-CoV-2 dynamics in WTD is warranted, and measures to minimize virus transmission between humans and animals are urgently needed.
登录
查看更多内容
影响因子：
6.7
作者：
Martins M;Boggiatto PM;Buckley A;Cassmann ED;Falkenberg S;Caserta LC;Fernandes MHV;Kanipe C;Lager K;Palmer MV;Diel DG
通讯作者：
Diel DG
影响因子：
28.3
作者：
Gorbalenya, Alexander E.;Baker, Susan C.;Ziebuhr, John
通讯作者：
Ziebuhr, John
影响因子：
168.9
作者：
Huang, Chaolin;Wang, Yeming;Cao, Bin
通讯作者：
Cao, Bin
影响因子：
3.9
作者：
Argimón S;Abudahab K;Goater RJE;Fedosejev A;Bhai J;Glasner C;Feil EJ;Holden MTG;Yeats CA;Grundmann H;Spratt BG;Aanensen DM
通讯作者：
Aanensen DM
影响因子：
2.6
作者：
Hanberry BB
通讯作者：
Hanberry BB