Effect of antibiotic pre-treatment and pathogen challenge on the intestinal microbiota in mice.
Effect of antibiotic pre-treatment and pathogen challenge on the intestinal microbiota in mice.
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DOI：
10.1186/s13099-016-0143-z
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发表时间：
2016
期刊：
影响因子：
4.2
通讯作者：
Blaser MJ
中科院分区：
文献类型：
--
作者：
Iizumi T;Taniguchi T;Yamazaki W;Vilmen G;Alekseyenko AV;Gao Z;Perez Perez GI;Blaser MJ
在抗生素发现50多年后，细菌感染已大幅减少;然而，抗生素也可能具有负面影响，例如增加对病原体的易感性。完整的微生物组是抵御病原体的重要防线。我们试图确定口服抗生素对病原体易感性和对微生物组的影响。我们研究了空肠弯曲杆菌，人类腹泻的最常见原因之一，以及鲍曼不动杆菌，导致伤口感染。我们研究了抗生素治疗对小鼠对这些病原体的易感性的影响以及它们对小鼠肠道微生物组的影响。在C57/BL 6小鼠模型中，我们探索了病原体攻击和抗生素治疗对肠道微生物群的影响。小鼠用环丙沙星、青霉素或水（对照）处理5天，然后在用C. jejuni或A.鲍曼不动杆菌，以评估抗生素对定植敏感性的影响。成功地将C. jejuni超过118天，但A.鲍曼不动杆菌。这些挑战没有对肠道微生物群的组成产生任何重大影响。虽然抗生素预处理没有改变病原体的定植，但它影响了肠道微生物组的丰富性和群落结构。然而，抗生素微生态失调显着减少病原体的挑战。我们的结论是，尽管肠道菌群干扰，这些病原体肠道定植的易感性是不变的。由抗生素治疗产生的主要肠道微生物组紊乱可以通过用特定微生物分类群定殖来减少。本文的在线版本（doi：10.1186/s13099-016-0143-z）包含补充材料，可供授权用户使用。
More than 50 years after the discovery of antibiotics, bacterial infections have decreased substantially; however, antibiotics also may have negative effects such as increasing susceptibility to pathogens. An intact microbiome is an important line of defense against pathogens. We sought to determine the effect of orally administered antibiotics both on susceptibility to pathogens and on impact to the microbiome. We studied Campylobacter jejuni, one of the most common causes of human diarrhea, and Acinetobacter baumannii, which causes wound infections. We examined the effects of antibiotic treatment on the susceptibility of mice to those pathogens as well as their influence on the mouse gut microbiome. In C57/BL6 mice models, we explored the effects of pathogen challenge, and antibiotic treatment on the intestinal microbiota. Mice were treated with either ciprofloxacin, penicillin, or water (control) for a 5-day period followed by a 5-day washout period prior to oral challenge with C. jejuni or A. baumannii to assess antibiotic effects on colonization susceptibility. Mice were successfully colonized with C. jejuni more than 118 days, but only transiently with A. baumannii. These challenges did not lead to any major effects on the composition of the gut microbiota. Although antibiotic pre-treatment did not modify pathogen colonization, it affected richness and community structure of the gut microbiome. However, the antibiotic dysbiosis was significantly reduced by pathogen challenge. We conclude that despite gut microbiota disturbance, susceptibility to gut colonization by these pathogens was unchanged. The major gut microbiome disturbance produced by antibiotic treatment may be reduced by colonization with specific microbial taxa. The online version of this article (doi:10.1186/s13099-016-0143-z) contains supplementary material, which is available to authorized users.
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影响因子：
6.4
作者：
BOHNHOFF, M;MILLER, CP
通讯作者：
MILLER, CP
DOI：
10.1038/nri3535
发表时间：
2013-11
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
12.2
作者：
Robinson CJ;Young VB
通讯作者：
Young VB
影响因子：
3.7
作者：
Chuang YC;Chang SC;Wang WK
通讯作者：
Wang WK
影响因子：
6.4
作者：
KORLATH, JA;OSTERHOLM, MT;ROBINSON, RA
通讯作者：
ROBINSON, RA