Surface-enhanced Raman scattering: An emerging tool for sensing cellular function.
Surface-enhanced Raman scattering: An emerging tool for sensing cellular function.
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表面增强拉曼散射：一种新兴的细胞功能传感工具。
DOI：
10.1002/wnan.1802
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发表时间：
2022-07
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对与关键细胞过程相关的生物分子变化进行连续长期细胞内成像和多重监测仍然是科学界面临的挑战。近年来，表面增强拉曼散射（Sers）技术以其显著的优势被证明是生物学领域中一种强有力的光谱工具。其中包括能够提供具有极高灵敏度的分子特异性信息、处理少量珍贵样品、真实的实时监测和最佳光学对比度。更重要的是，大量新型拉曼报告分子的光谱峰/振动模式的半峰全宽（FWHM）比传统荧光团更窄，这创造了一种多功能的基于Sers的探针，允许靶向多重传感，甚至超过荧光探针的检测灵敏度。由于其非破坏性的性质，它的适用性已被公认为生物传感，分子成像和动态监测复杂的细胞内过程。我们批判性地讨论了这一领域的最新发展，重点是不同的应用程序，其中Sers已被用于获得信息，仍然难以捉摸的传统成像方法。目前的报道表明，表面增强拉曼Sers在生物学领域取得了重大进展，并有可能用于人体体内的应用。诊断工具>基于纳米粒子的体外传感纳米技术生物学方法>生物学中的纳米尺度系统诊断工具>生物传感诊断工具>在体内纳米诊断和成像本文分类下：Sers是一种快速，超灵敏，非侵入性和多功能的技术，用于生物医学和诊断应用。
Continuous long‐term intracellular imaging and multiplexed monitoring of biomolecular changes associated with key cellular processes remains a challenge for the scientific community. Recently, surface‐enhanced Raman scattering (SERS) has been demonstrated as a powerful spectroscopic tool in the field of biology owing to its significant advantages. Some of these include the ability to provide molecule‐specific information with exquisite sensitivity, working with small volumes of precious samples, real‐time monitoring, and optimal optical contrast. More importantly, the availability of a large number of novel Raman reporters with narrower full width at half maximum (FWHM) of spectral peaks/vibrational modes than conventional fluorophores has created a versatile palette of SERS‐based probes that allow targeted multiplex sensing surpassing the detection sensitivity of even fluorescent probes. Due to its nondestructive nature, its applicability has been recognized for biological sensing, molecular imaging, and dynamic monitoring of complex intracellular processes. We critically discuss recent developments in this area with a focus on different applications where SERS has been used for obtaining information that remains elusive for conventional imaging methods. Current reports indicate that SERS has made significant inroads in the field of biology and has the potential to be used for in vivo human applications. Diagnostic Tools > In Vitro Nanoparticle‐Based Sensing Nanotechnology Approaches to Biology > Nanoscale Systems in Biology Diagnostic Tools > Biosensing Diagnostic Tools > In Vivo Nanodiagnostics and Imaging This article is categorized under: SERS is a rapid, ultrasensitive, noninvasive, and versatile technique for biomedical and diagnostics applications.
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