c-di-AMP binds the ydaO riboswitch in two pseudo-symmetry-related pockets.
c-di-AMP binds the ydaO riboswitch in two pseudo-symmetry-related pockets.
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DOI：
10.1038/nchembio.1606
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发表时间：
2014-09
影响因子：
14.8
通讯作者：
Patel, Dinshaw J.
中科院分区：
文献类型：
--
作者：
Ren, Aiming;Patel, Dinshaw J.
ydaO核开关参与孢子形成、渗透胁迫反应和细胞壁代谢，以亚纳摩尔亲和力靶向第二信使c-di-AMP。我们已经解决了c-di-AMP与热厌氧菌缩成ydaO核开关结合的结构，从而确定了一个包含拉链泡，假结和远程三级碱基对的五螺旋支架。重点包括在核开关的同一面上鉴定了两个c-二- amp结合袋，它们通过伪双重对称相关联，具有连接袋的邻接排列的碱基堆叠排列介导的位点间串音的潜力。结合的c-二磷酸腺苷分子的腺嘌呤环楔入碱基之间，并通过堆叠、碱基糖和糖-糖分子间氢键相互作用来稳定。结构研究补充了基于itc的突变体介导关键三级分子间接触的结合研究。与枯草芽孢杆菌类似，T. tengcongenesis的ydaO核糖开关可能通过转录终止机制起作用，c-di-AMP结合状态代表一个“关闭”开关。
The ydaO riboswitch, involved in sporulation, osmotic stress responses and cell wall metabolism, targets the second messenger c-di-AMP with subnanomolar affinity. We have solved the structure of c-di-AMP bound to the Thermoanaerobacter tengcongenesis ydaO riboswitch, thereby identifying a five-helical scaffold containing a zippered-up bubble, a pseudoknot and long-range tertiary base pairs. Highlights include the identification of two c-di-AMP binding pockets on the same face of the riboswitch, related by pseudo two-fold symmetry, with potential for cross-talk between sites mediated by adjacently-aligned base stacking alignments connecting pockets. The adenine rings of bound c-di-AMP molecules are wedged between bases and stabilized by stacking, base-sugar and sugar-sugar intermolecular hydrogen bonding interactions. The structural studies are complemented by ITC-based binding studies of mutants mediating key tertiary intermolecular contacts. The T. tengcongenesis ydaO riboswitch, like its B. subtilis counterpart, likely functions through a transcription termination mechanism, with the c-di-AMP bound state representing an ‘off’ switch.
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影响因子：
3.9
作者：
Smith KD;Strobel SA
通讯作者：
Strobel SA
DOI：
10.1073/pnas.1111701108
发表时间：
2011-09-06
影响因子：
11.1
作者：
Huang, Lili;Ishibe-Murakami, Satoko;Serganov, Alexander
通讯作者：
Serganov, Alexander
DOI：
10.1126/science.1159519
发表时间：
2008-07-18
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Sudarsan N;Lee ER;Weinberg Z;Moy RH;Kim JN;Link KH;Breaker RR
通讯作者：
Breaker RR
影响因子：
12.4
作者：
Serganov A;Patel DJ
通讯作者：
Patel DJ
影响因子：
64.8
作者：
Serganov, Alexander;Huang, Lili;Patel, Dinshaw J.
通讯作者：
Patel, Dinshaw J.