Hepatitis E Virus Detected in Pork Products
Hepatitis E Virus Detected in Pork Products
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猪肉制品检出戊型肝炎病毒
DOI：
10.1007/s12560-018-9354-4
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发表时间：
2018-08
影响因子：
3.4
通讯作者：
Yu Wenhai
中科院分区：
文献类型：
--
作者：
Hao Xianhui;Cao Wentao;Situ Jianwen;Zhao Yongqin;Huang Fen;Yu Wenhai
HEV感染是一个主要的全球公共卫生问题(Guera等人)。2017年)。HEV可以通过人畜传播给人类。猪被认为是HEV的主要宿主(Meng 2016)。进食生或未煮熟的猪肉或猪肝会导致急性HEV感染，这可能是世界上散发性HEV感染的主要来源(Okano等人。2014年)。最近，Moor et al.报告称，在瑞士的即食肉制品、肉制品(11.1%)、肝肠(18.9%)和生肉香肠(5.7%)中检测到HEV(Moor等人)。2018年)。然而，在传统上食用生猪肉和新鲜肝脏的云南省中国，缺乏关于猪的戊型肝炎病毒感染流行情况的信息。本研究用逆转录聚合酶链式反应(RT-PCR)检测了猪肉(n=57)、肝脏(n=12)、肠(n=16)、脾(n=3)、输尿管(n=19)等猪肉制品中HEV RNA。在57份零售猪肉中检出19份(33.3%)，12份零售猪肝中检出1份(8.3%)，16份肠道中检出3份(18.7%)，3份脾中检出1份(33.3%)，19份输尿管中检出5份(26.3%)(图1a)。由于样本有限，一些百分比可能只有相对意义。病毒滴度用基于SYBR Green的定量RT-PCR(qRT-PCR)和HEV特异的引物进行定量，如所述(Baylis等人。2013年)。病毒滴度：猪肉1.2×105拷贝/g~1.5×106拷贝/g，肠道2.3×105拷贝/g~1.7×106拷贝/g，肝脏约5.5×105拷贝/g，脾约8.3×105拷贝/g(图1b)。从猪肉制品中分离得到10株HEV的核苷酸序列，并登录GenBank，登录号为MF616013-MF616022。系统进化分析表明，所有从猪肉产品中分离的HEV毒株均属于HEV基因4h亚型，与人HEV的同源性为97.1-99.4%，与奶牛HEV的同源性为96.0-99.7%(图1c)。我们通过免疫组织化学分析进一步确认了这些猪肉产品中的HEV抗原(图1d)。HEV抗原的存在表明食用未经处理的猪肉产品有感染HEV的风险。总之，在传统食用生猪肉和新鲜肝脏的云南省中国零售猪肉产品中发现了HEVRNA的高流行率。人畜共患戊型肝炎病毒在猪与人之间传播的风险很高。
HEV infection is a main global public health issue (Guerra et al. 2017). HEV can zoonotically transmit to humans. Swine is recognized as a major reservoir of HEV (Meng 2016). Consuming raw or undercooked pork meat or pork liver leads to acute HEV infection, which may be the primary source of sporadic HEV infection in the world (Okano et al. 2014). Recently, Moor et al. reported that HEV was detected in ready-to-eat meat products, in meat products (11.1%), liver sausages (18.9%), and raw meat sausages (5.7%) in Switzerland (Moor et al. 2018). However, information is lacking on the prevalence of HEV infection in the swine in Yunnan province of China, where raw pork and fresh liver are consumed traditionally. In our study, the HEV RNA was investigated by reverse transcription PCR in retail pork products, including pork meat (n= 57), liver (n= 12), intestine (n= 16), spleen (n= 3), and ureter (n= 19). High prevalence of HEV RNA was found in 19 of total 57 (33.3%) in retail pork meat, 1 of 12 (8.3%) retail pig liver, 3 of 16 (18.7%) intestine, 1 of 3 (33.3%) spleen, and 5 of 19 (26.3%) ureter (Fig. 1 a). Because of the limited samples, some percentages might only be of relative significance. Viral titers were quantified using SYBR green-based quantitative RT-PCR (qRT-PCR) with HEV-specific primers as described (Baylis et al. 2013). The viral titers were 1.2× 105 copies/g to 1.5× 106 copies/g in pork meat, 2.3× 105 copies/g to 1.7× 106 copies/g in intestine, and approximately 5.5× 105 copies/g in the liver and 8.3× 105 copies/g in the spleen (Fig. 1 b). Ten nucleotide sequences of HEV isolated from pork products were obtained and deposited to GenBank under accession numbers MF616013–MF616022. Phylogenetic analysis shows that all HEV strains isolated from pork products belong to HEV genotype 4 and subtype 4 h with a 97.1–99.4% similarity to human HEV, and 96.0–99.7% to cow HEV (Fig. 1 c). We further confirmed the HEV antigens in these pork products by immunohistochemistry analysis (Fig. 1 d). The presence of HEV antigens suggested a risk of HEV infection upon consumption of untreated pork products. In summary, a high prevalence of HEV RNA was found in the retail pork products in Yunnan province of China, where raw pork and fresh liver are consumed traditionally. There is a high risk of zoonotic HEV transmission from swine to human.
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影响因子：
3.4
作者：
Moor D;Liniger M;Baumgartner A;Felleisen R
通讯作者：
Felleisen R
影响因子：
11.8
作者：
Baylis SA;Blümel J;Mizusawa S;Matsubayashi K;Sakata H;Okada Y;Nübling CM;Hanschmann KM;HEV Collaborative Study Group
通讯作者：
HEV Collaborative Study Group
影响因子：
3.6
作者：
Guerra JAAA;Kampa KC;Morsoletto DGB;Junior AP;Ivantes CAP
通讯作者：
Ivantes CAP
影响因子：
4.2
作者：
H. Okano;Masaharu Takahashi;Yoshiaki Isono;Hiroki Tanaka;T. Nakano;Yumi Oya;K. Sugimoto;Keiichi Ito;S. Ohmori;Tadashi Maegawa;Makoto Kobayashi;S. Nagashima;T. Nishizawa;H. Okamoto
通讯作者：
H. Okano;Masaharu Takahashi;Yoshiaki Isono;Hiroki Tanaka;T. Nakano;Yumi Oya;K. Sugimoto;Keiichi Ito;S. Ohmori;Tadashi Maegawa;Makoto Kobayashi;S. Nagashima;T. Nishizawa;H. Okamoto
影响因子：
6.7
作者：
Meng XJ
通讯作者：
Meng XJ