Biological effects of dosing aerobic exercise and neuromuscular electrical stimulation in rats.
Biological effects of dosing aerobic exercise and neuromuscular electrical stimulation in rats.
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DOI：
10.1038/s41598-017-11260-7
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发表时间：
2017-09-07
影响因子：
4.6
通讯作者：
Modo M
中科院分区：
文献类型：
--
作者：
Dalise S;Cavalli L;Ghuman H;Wahlberg B;Gerwig M;Chisari C;Ambrosio F;Modo M
有氧运动（AE）和非有氧神经肌肉电刺激（NMES）是物理治疗中常用的干预措施。我们探讨了这些干预措施对正常大鼠血液和大脑中的生化因子，如脑源性神经营养生长因子（BDNF），血管内皮生长因子-A（VEGF-A），胰岛素样生长因子-1（IGF-1）和Klotho的剂量依赖性（低，中，高），以及基于球磨机的最大容量试验（MCT）。中等剂量的AE使MCT改善最多，血液中Klotho呈剂量依赖性变化。在完成NMES方案后，BDNF的剂量依赖性增加是明显的，但MCT性能没有改善。AE和NMES后海马中的基因表达增加，IGF-1是与AE条件下MCT表现相关的信号分子，但也与VEGF-A和Klotho高度相关。血液Klotho水平可以作为AE治疗剂量的生物标志物，而IGF-1是与海马体中其他分子的基因表达偶联的关键分子。这种方法提供了一个可翻译的范例，以调查物理治疗中采用的干预措施的作用模式和机制，可以提高我们对这些因素在病理条件下如何变化的理解。
Aerobic exercise (AE) and non-aerobic neuromuscular electric stimulation (NMES) are common interventions used in physical therapy. We explored the dose-dependency (low, medium, high) of these interventions on biochemical factors, such as brain derived neurotrophic growth factor (BDNF), vascular endothelial growth factor-A (VEGF-A), insulin-like growth factor-1 (IGF-1) and Klotho, in the blood and brain of normal rats, as well as a treadmill-based maximum capacity test (MCT). A medium dose of AE produced the most improvement in MCT with dose-dependent changes in Klotho in the blood. A dose-dependent increase of BDNF was evident following completion of an NMES protocol, but there was no improvement in MCT performance. Gene expression in the hippocampus was increased after both AE and NMES, with IGF-1 being a signaling molecule that correlated with MCT performance in the AE conditions, but also highly correlated with VEGF-A and Klotho. Blood Klotho levels can serve as a biomarker of therapeutic dosing of AE, whereas IGF-1 is a key molecule coupled to gene expression of other molecules in the hippocampus. This approach provides a translatable paradigm to investigate the mode and mechanism of action of interventions employed in physical therapy that can improve our understanding of how these factors change under pathological conditions.
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影响因子：
3.6
作者：
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影响因子：
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作者：
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影响因子：
3.1
作者：
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通讯作者：
Abe K