Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen.
Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen.
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DOI：
10.1016/j.celrep.2018.07.080
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发表时间：
2018-08-21
期刊：
影响因子：
8.8
通讯作者：
Crispin M
中科院分区：
文献类型：
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作者：
Struwe WB;Chertova E;Allen JD;Seabright GE;Watanabe Y;Harvey DJ;Medina-Ramirez M;Roser JD;Smith R;Westcott D;Keele BF;Bess JW Jr;Sanders RW;Lifson JD;Moore JP;Crispin M
Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes. The same bnAbs also bind to recombinant, soluble trimeric immunogens based on the SOSIP design. While SOSIP trimers are close structural and antigenic mimics of virion Env, the extent to which their glycan structures resemble ones on infectious viruses is undefined. Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material. We also performed detailed site-specific analyses of gp120. Glycans relevant to key bnAb epitopes are generally similar on the recombinant SOSIP and virion-derived Env proteins, although the latter do contain hotspots of elevated glycan processing. Knowledge of native versus recombinant Env glycosylation will guide vaccine design and manufacturing programs. HIV envelope glycans are central features of broadly neutralizing antibody epitopes Recombinant mimetics of the HIV virus trimer are vaccine candidates Glycosylation of a leading trimer immunogen is similar to that of infectious virus Struwe et al. present site-specific analyses of N-glycosylation sites on HIV-1 envelope glycoproteins from an infectious virus and a recombinant trimer mimic. The structural and antigenic details of the glycan shield will be valuable for designing next-generation immunogens and understanding virus neutralization by broadly active antibodies.
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影响因子：
16.8
作者：
Kong, Leopold;Lee, Jeong Hyun;Doores, Katie J.;Murin, Charles D.;Julien, Jean-Philippe;McBride, Ryan;Liu, Yan;Marozsan, Andre;Cupo, Albert;Klasse, Per-Johan;Hoffenberg, Simon;Caulfield, Michael;King, C. Richter;Hua, Yuanzi;Le, Khoa M.;Khayat, Reza;Deller, Marc C.;Clayton, Thomas;Tien, Henry;Feizi, Ten;Sanders, Rogier W.;Paulson, James C.;Moore, John P.;Stanfield, Robyn L.;Burton, Dennis R.;Ward, Andrew B.;Wilson, Ian A.
通讯作者：
Wilson, Ian A.
影响因子：
82.9
作者：
通讯作者：
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DOI：
10.1084/jem.20161160
发表时间：
2017-09-04
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Medina-Ramírez M;Garces F;Escolano A;Skog P;de Taeye SW;Del Moral-Sanchez I;McGuire AT;Yasmeen A;Behrens AJ;Ozorowski G;van den Kerkhof TLGM;Freund NT;Dosenovic P;Hua Y;Gitlin AD;Cupo A;van der Woude P;Golabek M;Sliepen K;Blane T;Kootstra N;van Breemen MJ;Pritchard LK;Stanfield RL;Crispin M;Ward AB;Stamatatos L;Klasse PJ;Moore JP;Nemazee D;Nussenzweig MC;Wilson IA;Sanders RW
通讯作者：
Sanders RW
影响因子：
4.4
作者：
Go EP;Liao HX;Alam SM;Hua D;Haynes BF;Desaire H
通讯作者：
Desaire H
影响因子：
82.9
作者：
通讯作者：
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