Organ-specific remodeling of the Arabidopsis transcriptome in response to spaceflight.
Organ-specific remodeling of the Arabidopsis transcriptome in response to spaceflight.
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DOI：
10.1186/1471-2229-13-112
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发表时间：
2013-08-07
影响因子：
5.3
通讯作者：
Ferl RJ
中科院分区：
文献类型：
--
作者：
Paul AL;Zupanska AK;Schultz ER;Ferl RJ
太空飞行提供了一个不同于陆地生物进化经验的新环境。国际空间站作为一个科学平台的全面启动，配备了先进的植物生长室、实验室工作台和有效样本返回程序，使这一独特环境中的研究能力和假设检验达到了新的水平。有机会研究太空飞行中的环境传感策略（包括单位重力的缺失），为指导植物生长和发育的非生物因素（包括重力、光和触摸）之间的影响平衡提供了独特的见解。这里提供的数据将来自重复航天实验的形态学和转录组数据关联起来。拟南芥的转录组显示出响应太空飞行的器官特异性变化，与地面对照相比，太空飞行植物中有 480 个基因的表达发生了显着变化，变化至少为 1.9 倍，58 个基因变化超过 7 倍。叶子、下胚轴和根都表现出独特的反应模式，但反应中的许多基因功能是相关的。整个数据集中特别代表的是与细胞结构和生长激素信号传导相关的基因；预计微重力下不会改变的过程，但可能与航天植物中观察到的形态变化相关。例如，与触摸、细胞壁重塑、根毛和细胞扩张相关的基因的差异表达可能与太空飞行相关的根部倾斜相关，而生长素相关基因和其他重力信号基因的差异表达似乎与太空飞行的微重力相关。尽管功能相关的基因在叶子、下胚轴和根中的表达存在差异，但各个器官类型中各个基因的表达差异很大，这表明对太空飞行不存在单一的反应。相反，每个器官在共享策略中采用自己的反应策略，主要涉及细胞壁结构。太空飞行似乎启动了整个植物的细胞重塑，但植物特定器官之间的具体反应策略是不同的。此外，这些数据表明，在没有重力的情况下，植物依赖其他环境线索来启动对成功生长和发育至关重要的形态反应，而这种参与的基础在于基因以器官特异性方式的差异表达，从而最大限度地利用这些信号——例如与根部光感应相关的基因的上调。
Spaceflight presents a novel environment that is outside the evolutionary experience of terrestrial organisms. Full activation of the International Space Station as a science platform complete with sophisticated plant growth chambers, laboratory benches, and procedures for effective sample return, has enabled a new level of research capability and hypothesis testing in this unique environment. The opportunity to examine the strategies of environmental sensing in spaceflight, which includes the absence of unit gravity, provides a unique insight into the balance of influence among abiotic cues directing plant growth and development: including gravity, light, and touch. The data presented here correlate morphological and transcriptome data from replicated spaceflight experiments. The transcriptome of Arabidopsis thaliana demonstrated organ-specific changes in response to spaceflight, with 480 genes showing significant changes in expression in spaceflight plants compared with ground controls by at least 1.9-fold, and 58 by more than 7-fold. Leaves, hypocotyls, and roots each displayed unique patterns of response, yet many gene functions within the responses are related. Particularly represented across the dataset were genes associated with cell architecture and growth hormone signaling; processes that would not be anticipated to be altered in microgravity yet may correlate with morphological changes observed in spaceflight plants. As examples, differential expression of genes involved with touch, cell wall remodeling, root hairs, and cell expansion may correlate with spaceflight-associated root skewing, while differential expression of auxin-related and other gravity-signaling genes seemingly correlates with the microgravity of spaceflight. Although functionally related genes were differentially represented in leaves, hypocotyls, and roots, the expression of individual genes varied substantially across organ types, indicating that there is no single response to spaceflight. Rather, each organ employed its own response tactics within a shared strategy, largely involving cell wall architecture. Spaceflight appears to initiate cellular remodeling throughout the plant, yet specific strategies of the response are distinct among specific organs of the plant. Further, these data illustrate that in the absence of gravity plants rely on other environmental cues to initiate the morphological responses essential to successful growth and development, and that the basis for that engagement lies in the differential expression of genes in an organ-specific manner that maximizes the utilization of these signals – such as the up-regulation of genes associated with light-sensing in roots.
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影响因子：
3
作者：
Hohm, Tim;Preuten, Tobias;Fankhauser, Christian
通讯作者：
Fankhauser, Christian
影响因子：
6.9
作者：
Bosch M;Mayer CD;Cookson A;Donnison IS
通讯作者：
Donnison IS
影响因子：
5.6
作者：
Benatti MR;Penning BW;Carpita NC;McCann MC
通讯作者：
McCann MC
影响因子：
4.6
作者：
Hardtke, CS;Ckurshumova, W;Berleth, T
通讯作者：
Berleth, T
DOI：
10.1016/s0273-1177(02)00658-0
发表时间：
2003-01-01
期刊：
SPACE LIFE SCIENCES: MISSIONS TO MARS, RADIATION BIOLOGY, AND PLANTS AS A FOUNDATION FOR LONG-TERM LIFE SUPPORT SYSTEMS IN SPACE
影响因子：
--
作者：
Drysdale, AE;Ewert, MK;Hanford, AJ
通讯作者：
Hanford, AJ