Mammary adipocyte flow cytometry as a tool to study mammary gland biology.
Mammary adipocyte flow cytometry as a tool to study mammary gland biology.
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DOI：
10.1002/2211-5463.13620
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发表时间：
2023-07
期刊：
影响因子：
2.6
通讯作者：
中科院分区：
文献类型：
--
作者：
乳腺是哺乳动物中进化的重要外分泌器官，其分泌乳汁并提供营养以确保新生儿的生长和存活。小鼠乳腺在每次雌性经历妊娠和哺乳时显示出非凡的可塑性，包括三级分支和肺泡发生的复杂过程，以形成分支上皮树和随后的产乳肺泡。在泌乳停止后，腺体通过高度调节的退化过程重塑回到简单的导管结构。在细胞水平，可塑性的特征是乳腺细胞群的增殖，分化和凋亡，伴随着细胞功能和形态的重大变化。乳腺上皮需要特定的基质环境才能生长，称为乳腺脂肪垫。乳腺脂肪细胞是脂肪垫中最突出的细胞类型之一，但是尽管它们在组织中占很大比例并且它们与上皮细胞的相互作用至关重要，但它们的生理学在很大程度上仍然未知。在过去的十年中，了解乳腺脂肪细胞的性质和贡献的必要性已得到更多的认可。然而，研究这种细胞生态位的适当方法和方案的发展仍然滞后，部分原因是它们的脆弱性，分离它们的困难，缺乏可靠的细胞表面标记物和这种组织中的异质环境，这与其他脂肪细胞库不同。在这里，我们描述了一种新的快速和简单的流式细胞术协议，专门设计用于分析和分离小鼠乳腺脂肪细胞在乳腺发育阶段。乳腺脂肪细胞是一个研究不足的细胞生态位与哺乳和内分泌信号的重要影响。这种组织的动态和细胞异质性使其研究具有挑战性。我们已经建立了一个新的高效和简单的流式细胞术协议，专门设计用于分析和分离小鼠乳腺脂肪细胞在整个乳腺发育周期。
The mammary gland is a vital exocrine organ that has evolved in mammals to secrete milk and provide nutrition to ensure the growth and survival of the neonate The mouse mammary gland displays extraordinary plasticity each time the female undergoes pregnancy and lactation, including a sophisticated process of tertiary branching and alveologenesis to form a branched epithelial tree and subsequently milk‐producing alveoli. Upon the cessation of lactation, the gland remodels back to a simple ductal architecture via highly regulated involution processes. At the cellular level, the plasticity is characterised by proliferation of mammary cell populations, differentiation and apoptosis, accompanied by major changes in cell function and morphology. The mammary epithelium requires a specific stromal environment to grow, known as the mammary fat pad. Mammary adipocytes are one of the most prominent cell types in the fat pad, but despite their vast proportion in the tissue and their crucial interaction with epithelial cells, their physiology remains largely unknown. Over the past decade, the need to understand the properties and contribution of mammary adipocytes has become more recognised. However, the development of adequate methods and protocols to study this cellular niche is still lagging, partially due to their fragile nature, the difficulty of isolating them, the lack of reliable cell surface markers and the heterogenous environment in this tissue, which differs from other adipocyte depots. Here, we describe a new rapid and simple flow cytometry protocol specifically designed for the analysis and isolation of mouse mammary adipocytes across mammary gland developmental stages. Mammary adipocytes are an understudied cellular niche with important implications for lactation and endocrine signalling. The dynamic and cellular heterogeneity of this tissue makes it challenging to study. We have established a new efficient and simple flow cytometry protocol specifically designed for the analysis and isolation of mouse mammary adipocytes throughout the developmental cycle of the mammary gland.
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影响因子：
2.5
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Hovey, Russell C.;Aimo, Lucila
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Aimo, Lucila
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Horsley V