Alterations in Oral-Nasal-Pharyngeal Microbiota and Salivary Proteins in Mouth-Breathing Children.
Alterations in Oral-Nasal-Pharyngeal Microbiota and Salivary Proteins in Mouth-Breathing Children.
复制标题
用口呼吸的儿童口腔-鼻腔-咽部微生物群和唾液蛋白质的变化
DOI：
10.3389/fmicb.2020.575550
复制
发表时间：
2020
影响因子：
5.2
通讯作者：
Lin H
中科院分区：
文献类型：
--
作者：
Fan C;Guo L;Gu H;Huo Y;Lin H
口腔呼吸可诱发一系列疾病，但对口腔微生物群和唾液蛋白的影响尚不清楚。本研究首次采用16S核糖体DNA (rDNA) （V3-V4区）高通量测序技术，对有口呼吸患儿（MB组，n = 10）与配对有鼻呼吸患儿（NB组，n = 10）的口鼻咽微生物群进行比较。采用无标记定量（label-free quantitation， LFQ）方法分析差异表达的唾液蛋白，并采用典型对应分析（canonical correspondence analysis， CCA）方法分析差异表达蛋白与细菌丰度的关系。两组的总体细菌分布不同，这种差异与口腔呼吸的持续时间有关。口腔-咽菌群多样性显著增高，鼻-咽菌种趋于一致（未加权UniFrac， p = 0.38）。机会致病菌相对丰度较高的有：前龈上菌斑中的不动杆菌、未刺激唾液中的奈瑟菌、咽中的肺炎链球菌和鼻孔中的窄养单胞菌。MB组唾液氧化应激相关蛋白（乳酸谷胱甘肽裂解酶和过氧化物还蛋白-5）表达上调，免疫相关蛋白（整合素- m和蛋白酶体亚基α -1型）表达下调。差异表达蛋白与特定细菌相关，表明它们有潜力作为诊断、早期干预和口腔呼吸治疗靶点的候选生物标志物。本研究表明，口腔呼吸影响口腔-鼻-咽微生物群，丰富某些病原体，并伴有唾液环境的改变。进一步研究病理机制和动态变化的纵向研究是必要的。
Mouth breathing induces a series of diseases, while the influence on microbiota of oral cavity and salivary proteins remains unknown. In this study, for the first time, profiles of oral–nasal–pharyngeal microbiota among mouth-breathing children (MB group, n = 10) were compared with paired nose-breathing children (NB group, n = 10) using 16S ribosomal DNA (rDNA) (V3–V4 region) high-throughput sequencing. The differentially expressed salivary proteins were revealed using label-free quantification (LFQ) method, and their associations with bacterial abundance were measured by canonical correspondence analysis (CCA). The overall bacterial profiles differed between the two groups, and the differences were related to the duration of mouth breathing. The diversity of oral–pharyngeal microbiota was significantly higher, and the nasal–pharyngeal species tended to be consistent (unweighted UniFrac, p = 0.38) in the MB group. Opportunistic pathogens were higher in relative abundance as follows: Acinetobacter in the anterior supragingival plaque, Neisseria in unstimulated saliva, Streptococcus pneumoniae in the pharynx, and Stenotrophomonas in the nostrils. The expression level of oxidative-stress-related salivary proteins (lactoylglutathione lyase and peroxiredoxin-5) were upregulated, while immune-related proteins (integrin alpha-M and proteasome subunit alpha type-1) were downregulated in MB group. The differentially expressed proteins were associated with specific bacteria, indicating their potentials as candidate biomarkers for the diagnosis, putatively early intervention, and therapeutic target of mouth breathing. This study showed that mouth breathing influences the oral–nasal–pharyngeal microbiota and enriches certain pathogens, accompanied with the alterations in the salivary environment. Further research on the pathological mechanisms and dynamic changes in longitudinal studies are warranted.
登录
查看更多内容
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.33594/000000117
发表时间：
2019-01-01
期刊：
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
影响因子：
--
作者：
Akhter, Nadeem;Madhoun, Ashraf;Sindhu, Sardar
通讯作者：
Sindhu, Sardar
影响因子：
4.6
作者：
Huang CC;Wu PW;Chen CL;Wang CH;Lee TJ;Tsai CN;Chiu CH
通讯作者：
Chiu CH
影响因子：
21.1
作者：
Gao L;Xu T;Huang G;Jiang S;Gu Y;Chen F
通讯作者：
Chen F
DOI：
10.1016/j.oooo.2013.07.013
发表时间：
2013-11-01
影响因子：
2.9
作者：
Feller, L.;Altini, M.;Lemmer, J.
通讯作者：
Lemmer, J.