Genomic Analysis of Aeromonas veronii C198, a Novel Mcr-3.41-Harboring Isolate from a Patient with Septicemia in Thailand.
Genomic Analysis of Aeromonas veronii C198, a Novel Mcr-3.41-Harboring Isolate from a Patient with Septicemia in Thailand.
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泰国败血症患者携带MCR-3.41基因的新型韦氏气单胞菌C198的基因组分析
DOI：
10.3390/pathogens9121031
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发表时间：
2020-12-09
期刊：
影响因子：
--
通讯作者：
Hamada S
中科院分区：
文献类型：
--
作者：
Hatrongjit R;Kerdsin A;Takeuchi D;Wongsurawat T;Jenjaroenpun P;Chopjitt P;Boueroy P;Akeda Y;Hamada S
由质粒携带的mcr基因介导的革兰氏阴性菌对粘菌素的耐药性是一个新出现的公共卫生问题。使用短读码和长读码平台确定从败血症患者获得的Veronii生物变种Veronii气单胞菌临床分离株的全基因组序列（4.55 Mb）。发现该分离株（C198）携带一种新型mcr-3基因，命名为mcr-3.41。分离株C198显示相邻的mcr-3.41和mcr-3样基因。它含有一条染色体和两个质粒，两者都编码RepB复制蛋白。还存在其他抗菌素耐药基因，包括blacphA 3、blaOXA-12、tetA、rsmA和adeF。C198株对阿莫西林-克拉维酸、氨苄西林-舒巴坦和四环素耐药，对甲氧苄啶-磺胺甲恶唑中度耐药。该菌株对哌拉西林-他唑巴坦、碳青霉烯、第三代头孢菌素、氟喹诺酮类、氯霉素和氨基糖苷类药物敏感。C198基因组中的推定毒力基因编码II型、III型和VI型分泌系统; IV型气单胞菌皮利;和I型菌毛、鞭毛、血凝素、气单胞菌溶血素和溶血素。多位点序列分型揭示了一种新的序列类型（ST），C198的ST 720。系统发育分析表明，C198菌株与A. veronii 17ISAe.本研究为人类A. Veronii分离物。
The resistance of Gram-negative bacteria to colistin, mediated by plasmid-borne mcr genes, is an emerging public health concern. The complete genome sequence (4.55 Mb) of a clinical isolate of Aeromonas veronii biovar veronii obtained from a patient with septicemia was determined using short-read and long-read platforms. This isolate (C198) was found to harbor a novel mcr-3 gene, designated mcr-3.41. Isolate C198 revealed adjacent mcr-3.41 and mcr-3-like genes. It contained one chromosome and two plasmids, both of which encoded a RepB replication protein. Other antimicrobial resistance genes, including blacphA3, blaOXA-12, tetA, rsmA, and adeF, were also present. Isolate C198 was resistant to amoxicillin–clavulanate, ampicillin–sulbactam and tetracycline, and showed intermediate resistance to trimethoprim–sulfamethoxazole. The isolate was susceptible to piperacillin–tazobactam, carbapenem, third-generation cephalosporins, fluoroquinolones, chloramphenicol, and aminoglycosides. Putative virulence genes in the C198 genome encoded type II, III, and VI secretion systems; type IV Aeromonas pili; and type I fimbria, flagella, hemagglutinin, aerolysin, and hemolysins. Multilocus sequence typing revealed a novel sequence type (ST), ST720 for C198. Phylogenetic analysis of the single nucleotide polymorphisms in C198 demonstrated that the strain was closely related to A. veronii 17ISAe. The present study provides insights into the genomic characteristics of human A. veronii isolates.
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作者：
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发表时间：
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