Quantitative Peptidomics of Mouse Brain After Infection With Cyst-Forming Toxoplasma gondii.
Quantitative Peptidomics of Mouse Brain After Infection With Cyst-Forming Toxoplasma gondii.
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DOI：
10.3389/fimmu.2021.681242
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发表时间：
2021
影响因子：
7.3
通讯作者：
Zhou HY
中科院分区：
文献类型：
--
作者：
Zhou CX;Gao M;Han B;Cong H;Zhu XQ;Zhou HY
弓形虫是一种专性细胞内寄生虫，能够在宿主脑内建立持续感染并诱导严重的神经病理学。肽是中枢神经系统中重要的天然分子，在中枢神经系统中具有广泛的生物学功能。从未对弓形虫感染进行过调查。使用无标记肽组学方法（LC-MS/MS），我们从急性感染、慢性感染和对照脑样品中鉴定了总共2，735种内源性肽。弓形虫感染定量分析显示，急性期和慢性期差异表达肽（DEPs）分别为478个和344个，利用Gene Ontology对DEPs进行功能分析，发现这些DEPs主要来源于细胞部分，参与细胞过程。我们还确定了三个新的神经肽来源于前体蛋白胆囊收缩素。这些结果表明，定量肽组学在确定生物活性肽和阐明其在T。弓形虫感染
Toxoplasma gondii is an obligate intracellular parasite capable of establishing persistent infection within the host brain and inducing severe neuropathology. Peptides are important native molecules responsible for a wide range of biological functions within the central nervous system. However, peptidome profiling in host brain during T. gondii infection has never been investigated. Using a label-free peptidomics approach (LC–MS/MS), we identified a total of 2,735 endogenous peptides from acutely infected, chronically infected and control brain samples following T. gondii infection. Quantitative analysis revealed 478 and 344 significantly differentially expressed peptides (DEPs) in the acute and chronic infection stages, respectively. Functional analysis of DEPs by Gene Ontology suggested these DEPs mainly originated from cell part and took part in cellular process. We also identified three novel neuropeptides derived from the precursor protein cholecystokinin. These results demonstrated the usefulness of quantitative peptidomics in determining bioactive peptides and elucidating their functions in the regulation of behavior modification during T. gondii infection.
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DOI：
10.9758/cpn.2018.16.1.95
发表时间：
2018-02-28
期刊：
Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology
影响因子：
--
作者：
Bak J;Shim SH;Kwon YJ;Lee HY;Kim JS;Yoon H;Lee YJ
通讯作者：
Lee YJ
影响因子：
9.3
作者：
Lang D;Schott BH;van Ham M;Morton L;Kulikovskaja L;Herrera-Molina R;Pielot R;Klawonn F;Montag D;Jänsch L;Gundelfinger ED;Smalla KH;Dunay IR
通讯作者：
Dunay IR
DOI：
10.1074/jbc.m115.703066
发表时间：
2016-05-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Liu K;Li F;Han H;Chen Y;Mao Z;Luo J;Zhao Y;Zheng B;Gu W;Zhao W
通讯作者：
Zhao W
影响因子：
14.9
作者：
Ma J;Chen T;Wu S;Yang C;Bai M;Shu K;Li K;Zhang G;Jin Z;He F;Hermjakob H;Zhu Y
通讯作者：
Zhu Y
影响因子：
3.5
作者：
Diaz, Francisca;Garcia, Sofia;Moraes, Carlos T.
通讯作者：
Moraes, Carlos T.