Aberrant accumulation of p53 associates with Ki67 and mitotic count in benign skin lesions
Aberrant accumulation of p53 associates with Ki67 and mitotic count in benign skin lesions
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p53 的异常积累与良性皮肤病变中的 Ki67 和有丝分裂计数相关
DOI：
10.1111/j.1365-2133.1994.tb08552.x
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发表时间：
1994
影响因子：
10.3
通讯作者：
K. Vähäkangas
中科院分区：
文献类型：
--
作者：
Y. Soini;D. Kamel;P. Pääkkö;V. Lehto;A. Oikarinen;K. Vähäkangas
采用免疫组织化学染色方法，用p53多克隆抗体（CM‐1）和Ki67单克隆抗体对62例不同良性疾病（20例银屑病、14例慢性皮炎、11例脂湿性角化病、11例扁平苔藓）和7例正常皮肤样本进行染色，在大多数病变中发现p53阳性角质形成细胞。然而，p53阳性细胞的百分比远低于p53阳性恶性肿瘤。正常皮肤样品未见p53反应性。在所有皮肤样本中观察到可变的Ki67反应性。总体而言，在p53阳性细胞比例较高的皮肤样本中，Ki67阳性细胞的数量较高（占表皮细胞总数的0.5%）（P=0.004）。这也分别适用于银屑病和非银屑病病变（P=0.028和P=0.033分别）。在Ki67阳性细胞中>占10%的情况下，有丝分裂明显增加（P<0.001）。这种关联适用于银屑病和研究的其他病变（P分别=0.024和P <0.001）。结果表明，免疫组织化学检测到p53的积累是在非肿瘤性皮肤病变中经常发现的。由于p53阳性与增殖标志物Ki67相关，p53的积累可能是对这些皮肤病中角质形成细胞增殖率增加的反应，或者它可能与细胞凋亡有关。
Sixty‐two skin samples from patients with a variety of benign disorders (20 cases of psoriasis, 14 cases of chronic dermatitis, 11 seborrhoeic keratoses, 11 cases of lichen planus), and seven normal skin samples, were stained immunohistochemically with a polyclonal antibody (CM‐1) to p53, and a monoclonal antibody to Ki67, using the avidin‐biotin complex method, p53‐positive keratinocytes could be found in most of these lesions. The percentage of p53‐positive cells was, however, far lower than usually seen in p53‐positive malignant tumours. No p53 reactivity was observed in the normal skin samples. Variable Ki67 reactivity was observed in all skin samples. Overall, the number of Ki67‐positive cells was higher in skin samples in which the proportion of p53‐positive cells was high (>0.5% of total epidermal cell population) (P=0.004). This also applied separately to psoriatic and non‐psoriatic lesions (P=0.028 and P=0.033, respectively). In cases with >10% of Ki67‐positive cells, there were significantly more mitoses (P<0.001). This association applied to both psoriasis and the other lesions studied (P=0.024 and P <0.001, respectively). The results show that immunohistochemically detectable accumulation of p53 is a frequent finding in non‐neoplastic skin lesions. As p53 positivity was associated with the proliferation marker Ki67, the accumulation of p53 is possibly a response to an increased proliferation rate of the keratinocytes in these skin diseases, or alternatively it may be associated with apoptosis.
影响因子：
8
作者：
R. Maestro;R. Dolcetti;D. Gasparotto;C. Doglioni;S. Pelucchi;L. Barzan;E. Grandi;M. Boiocchi
通讯作者：
R. Maestro;R. Dolcetti;D. Gasparotto;C. Doglioni;S. Pelucchi;L. Barzan;E. Grandi;M. Boiocchi
DOI：
10.1073/pnas.88.5.1958
发表时间：
1991-03-01
影响因子：
11.1
作者：
MERCER, WE;SHIELDS, MT;ULLRICH, SJ
通讯作者：
ULLRICH, SJ
DOI：
10.1073/pnas.88.22.10124
发表时间：
1991-11-01
影响因子：
11.1
作者：
BRASH, DE;RUDOLPH, JA;PONTEN, J
通讯作者：
PONTEN, J