New approaches with different types of circulating cathodic antigen for the diagnosis of patients with low Schistosoma mansoni load.
New approaches with different types of circulating cathodic antigen for the diagnosis of patients with low Schistosoma mansoni load.
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DOI：
10.1371/journal.pntd.0002054
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发表时间：
2013
影响因子：
3.8
通讯作者：
Coelho PM
中科院分区：
文献类型：
--
作者：
Grenfell R;Harn DA;Tundup S;Da'dara A;Siqueira L;Coelho PM
Schistosomiasis mansoni is a debilitating and sometimes fatal disease. Accurate diagnosis plays a key role in patient management and infection control. However, currently available parasitological methods are laborious and lack sensitivity. The selection of target antigen candidates has turned out to be a promising tool for the development of more sensitive diagnostic methods. In our previous investigations, the use of crude antigens led to false-positive results. Recently, focus has been given to highly purified Schistosoma mansoni antigens, especially to circulating antigens. Thus, our main goal was to test different types of circulating cathodic antigen glycoprotein (CCA), as “crude antigen,” the protein chain of recombinant CCA and two individual peptides. These schistosome proteins/peptides were tested in a new diagnostic method employing immunomagnetic separation based on the improvement of antigen–antibody binding. Use of recombinant CCA as a diagnostic antigen allowed us to develop a diagnostic assay with high sensitivity and specificity with no false-negative results. Interestingly, the “crude antigen” worked as a good marker for control of cure after praziquantel treatment. Our new diagnostic method was superior to enzyme-linked immunosorbent assay in diagnosing low endemicity patients. Schistosomiasis mansoni is a debilitating and sometimes fatal disease that affects many individuals in Africa and Brazil. Currently available diagnostic methods are not sensitive for patients with low parasite load which leads to underreported cases. The selection of target diagnostic antigen candidates is a promising tool for the development of a new and more sensitive assay. In this study, we focused on different types of circulating cathodic antigen (CCA) for development of an innovative assay. This new assay is called immunomagnetic separation and it uses magnetic microspheres attached to the antigens in order to improve the diagnostic sensitivity. Best results were found when we used the protein chain of the recombinant CCA showing high sensitivity and specificity with no false-negative results. Together, the use of “crude antigen” presented good results for the control of cure. Our new assay was superior to enzyme-linked immunosorbent assay in discriminating positive and negative cases, especially related to patients with low parasite load.
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影响因子：
3.1
作者：
Elhag, Salma Medani;Abdelkareem, Eshtiyag Abdalla;Mohamed, Ahmed Babiker
通讯作者：
Mohamed, Ahmed Babiker
影响因子：
2.7
作者：
FILLIE, YE;VANLIESHOUT, L;DEELDER, AM
通讯作者：
DEELDER, AM
DOI：
10.1007/bf00928334
发表时间：
1985-01-01
期刊：
ZEITSCHRIFT FUR PARASITENKUNDE-PARASITOLOGY RESEARCH
影响因子：
--
作者：
DEELDER, AM;ELDOSOKY, I;QIAN, ZL
通讯作者：
QIAN, ZL
DOI：
10.1016/0035-9203(90)90094-u
发表时间：
1990-11-01
影响因子：
2.2
作者：
DEJONGE, N;KREMSNER, PG;DEELDER, AM
通讯作者：
DEELDER, AM
影响因子：
2.7
作者：
El-Manzalawy, Yasser;Dobbs, Drena;Honavar, Vasant
通讯作者：
Honavar, Vasant