Transcriptome analysis of Pinus halepensis under drought stress and during recovery.
Transcriptome analysis of Pinus halepensis under drought stress and during recovery.
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DOI：
10.1093/treephys/tpx137
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发表时间：
2018-03-01
期刊：
影响因子：
4
通讯作者：
David-Schwartz R
中科院分区：
文献类型：
--
作者：
Fox H;Doron-Faigenboim A;Kelly G;Bourstein R;Attia Z;Zhou J;Moshe Y;Moshelion M;David-Schwartz R
森林树木采用各种策略来应对干旱胁迫，这些策略涉及复杂的分子机制。阿勒颇松（Pinus halepensis Miller）遍布地中海盆地，是最耐旱的松树物种之一。为了破译P. halepensis抵御干旱的分子机制，我们进行了大规模的生理和转录组分析。我们选择了一棵生长条件不理想的半干旱地区的成熟树木，通过扦插进行无性系繁殖。然后，我们使用高通量实验系统连续监测全株蒸腾速率、气孔导度和蒸汽压亏缺。研究了植物在气孔前响应、气孔部分关闭、最小蒸腾、灌后、部分恢复和完全恢复6个生理阶段的转录组。在每个阶段，将干旱处理植株的数据与灌溉良好的对照植株的数据进行比较。利用对端rna测序技术构建了干旱胁迫下的halepensis转录组。在干旱处理树和对照树之间共鉴定出约6000个差异表达的非冗余转录本。聚类分析显示，胁迫诱导的转录本下调与光合作用、通过抗坏血酸(AsA)-谷胱甘肽循环清除活性氧（ROS）、脂肪酸和细胞壁生物合成、气孔活性以及类黄酮和萜类化合物的生物合成有关。上调的过程包括叶绿素降解、通过不依赖asa的硫醇介导途径清除ros、脱落酸反应和热休克蛋白、thaumatin和exordium的积累。干旱恢复诱导逆转录转座子的强转录，特别是逆转录病毒相关转座子Tnt1-94。干旱相关转录组说明了该物种对干旱和恢复的动态响应，并揭示了新的机制。
Forest trees use various strategies to cope with drought stress and these strategies involve complex molecular mechanisms. Pinus halepensis Miller (Aleppo pine) is found throughout the Mediterranean basin and is one of the most drought-tolerant pine species. In order to decipher the molecular mechanisms that P. halepensis uses to withstand drought, we performed large-scale physiological and transcriptome analyses. We selected a mature tree from a semi-arid area with suboptimal growth conditions for clonal propagation through cuttings. We then used a high-throughput experimental system to continuously monitor whole-plant transpiration rates, stomatal conductance and the vapor pressure deficit. The transcriptomes of plants were examined at six physiological stages: pre-stomatal response, partial stomatal closure, minimum transpiration, post-irrigation, partial recovery and full recovery. At each stage, data from plants exposed to the drought treatment were compared with data collected from well-irrigated control plants. A drought-stressed P. halepensis transcriptome was created using paired-end RNA-seq. In total, ~6000 differentially expressed, non-redundant transcripts were identified between drought-treated and control trees. Cluster analysis has revealed stress-induced down-regulation of transcripts related to photosynthesis, reactive oxygen species (ROS)-scavenging through the ascorbic acid (AsA)-glutathione cycle, fatty acid and cell wall biosynthesis, stomatal activity, and the biosynthesis of flavonoids and terpenoids. Up-regulated processes included chlorophyll degradation, ROS-scavenging through AsA-independent thiol-mediated pathways, abscisic acid response and accumulation of heat shock proteins, thaumatin and exordium. Recovery from drought induced strong transcription of retrotransposons, especially the retrovirus-related transposon Tnt1-94. The drought-related transcriptome illustrates this species’ dynamic response to drought and recovery and unravels novel mechanisms.
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影响因子：
7.4
作者：
Clement, Mathilde;Leonhardt, Nathalie;Noel, Laurent D.
通讯作者：
Noel, Laurent D.
影响因子：
6.4
作者：
Chang, SJ;Puryear, JD;Cairney, J
通讯作者：
Cairney, J
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
16.6
作者：
Armbruster, Ute;Carrillo, L. Ruby;Venema, Kees;Pavlovic, Lazar;Schmidtmann, Elisabeth;Kornfeld, Ari;Jahns, Peter;Berry, Joseph A.;Kramer, David M.;Jonikas, Martin C.
通讯作者：
Jonikas, Martin C.
影响因子：
2.1
作者：
Caverzan A;Passaia G;Rosa SB;Ribeiro CW;Lazzarotto F;Margis-Pinheiro M
通讯作者：
Margis-Pinheiro M