The impact of acute stress disorder on gallbladder interstitial cells of Cajal
The impact of acute stress disorder on gallbladder interstitial cells of Cajal
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急性应激障碍对Cajal胆囊间质细胞的影响
DOI：
10.1002/jcp.29686
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发表时间：
2020-04
影响因子：
5.6
通讯作者：
Yu Bao-ping
中科院分区：
文献类型：
--
作者：
Huang Zhen-peng;Qiu Hu;Wang Ke;Chao Wei-bo;Zhu Hao-bin;Chen Hang;Liu Yue;Yu Bao-ping
身体和心理应激对胃肠动力障碍有很大影响，创伤会加重消化功能障碍的症状。Cajal间质细胞(ICCs)作为胃肠动力调节的起搏器，在应激性胃肠动力紊乱中可能起重要作用。本研究采用兔胸部穿刺术和胆囊切除术模型，探讨了急性应激条件下胆囊胞内皮细胞功能的机制。干细胞因子(SCF)/c-kit通路对于ICCs的发育是必不可少的，基因表达被研究以确定应激诱导的转录变化。免疫组织化学、末端脱氧核苷酸转移酶dUTP缺口末端标记法检测ICCs的凋亡，Western印迹分析和逆转录聚合酶链式反应检测SCF/c-kit信号通路的变化。这些方法显示应激后ICCs通过细胞凋亡减少，去除应激源后ICCs随着时间的推移而增加。因此，本研究证实了应激对ICCs发育和存活的影响，并进一步证实了应激与胃肠动力之间的联系。
Physical and psychological stress exerts a substantial effect on gastrointestinal motility disorders, where trauma enhances symptoms of digestive dysfunction. Interstitial cells of Cajal (ICCs) act as pacemakers for gastrointestinal motility regulation and are likely important in stress‐associated gastrointestinal motility disorders. This study explored the mechanisms underlying gallbladder ICCs function under acute stress conditions using a rabbit chest puncture and cholecystectomy model. The stem cell factor (SCF)/c‐kit pathway is essential for the development of ICCs, and gene expression was investigated to identify stress‐induced transcriptional alterations. Immunohistochemistry, terminal deoxynucleotidyl transferase dUTP nick end labeling assays were used to determine ICCs apoptosis, whereas western blot analysis and reverse‐transcription polymerase chain reaction were used to detect changes in the SCF/c‐kit signaling pathway. These methods revealed a reduction in ICCs via apoptosis following stress, and ICCs increased over time after stressor removal. Therefore, this study demonstrates the impact of stress on ICCs development and survival and further confirms the link between stress and gastrointestinal motility.
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影响因子：
4.4
作者：
Dai W;Liu A;Kaminga AC;Deng J;Lai Z;Yang J;Wen SW
通讯作者：
Wen SW
影响因子：
3.5
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Gibbons SJ;De Giorgio R;Faussone Pellegrini MS;Garrity-Park MM;Miller SM;Schmalz PF;Young-Fadok TM;Larson DW;Dozois EJ;Camilleri M;Stanghellini V;Szurszewski JH;Farrugia G
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Fan, Ying;Wu, Shuodong;Wang, Xinpeng
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Wang, Xinpeng
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作者：
B. T. Johnston;R. J. Mcfarland;J. Collins;A. H. Love
通讯作者：
B. T. Johnston;R. J. Mcfarland;J. Collins;A. H. Love