Apoptotic cell death of human interstitial cells of Cajal.
Apoptotic cell death of human interstitial cells of Cajal.
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DOI：
10.1111/j.1365-2982.2008.01185.x
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发表时间：
2009-01
影响因子：
3.5
通讯作者：
Farrugia G
中科院分区：
文献类型：
--
作者：
Gibbons SJ;De Giorgio R;Faussone Pellegrini MS;Garrity-Park MM;Miller SM;Schmalz PF;Young-Fadok TM;Larson DW;Dozois EJ;Camilleri M;Stanghellini V;Szurszewski JH;Farrugia G
Cajal间质细胞(ICC)是一种特殊的间质来源的细胞，它调节许多平滑肌的收缩和兴奋性，在各种肠道动力障碍中可见ICC的丢失。ICC数量的维持受到严格的控制，有几个已知的因素可以调节增殖。相比之下，国际刑事法院的命运尚未确定。本研究的目的是探讨细胞凋亡是否在正常结肠ICC数量的调节中起作用。通过c-Kit受体酪氨酸激酶免疫标记和电子显微镜鉴定ICC。免疫组织化学方法检测caspase-3活性、dUTP末端末端标记及细胞超微结构改变，检测结肠组织细胞凋亡情况。在双标记组织切片中检测和计数凋亡的ICC。结肠肌各层均可见凋亡的ICC。在固有肌中，1.5±0.2%的ICC表达活化的caspase-3，在环肌层中，2.1±0.9%的ICC表达TUNEL。电子显微镜下可见凋亡的ICC。在ICC中，细胞凋亡性死亡正在进行。健康结肠ICC中的细胞凋亡水平表明，这些细胞必须不断再生才能维持完整的网络。
Interstitial cells of Cajal (ICC) are specialized mesenchyme-derived cells that regulate contractility and excitability of many smooth muscles with loss of ICC seen in a variety of gut motility disorders. Maintenance of ICC numbers is tightly regulated, with several factors known to regulate proliferation. In contrast, the fate of ICC is not established. The aim of this study was to investigate whether apoptosis plays a role in the regulation of ICC numbers in the normal colon. ICC were identified by immunolabeling for the c-Kit receptor tyrosine kinase and by electron microscopy. Apoptosis was detected in colon tissue by immunolabeling for activated caspase-3, terminal dUTP nucleotide end labeling, and ultrastructural changes in the cells. Apoptotic ICC were identified and counted in double labeled tissue sections. Apoptotic ICC were identified in all layers of the colonic muscle. In the muscularis propria 1.5 ± 0.2% of ICC were positive for activated caspase-3 and in the circular muscle layer 2.1 ± 0.9% of ICC were positive for TUNEL. Apoptotic ICC were identified by electron microscopy. Apoptotic cell death is ongoing in ICC. The level of apoptosis in ICC in healthy colon indicates that these cells must be continually regenerated to maintain intact networks.
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影响因子：
11.2
作者：
Hacker, E;Muller, HK;Walker, G
通讯作者：
Walker, G
DOI：
10.1073/pnas.1431233100
发表时间：
2003-07-08
影响因子：
11.1
作者：
Farrugia, G;Lei, S;Szurszewski, JH
通讯作者：
Szurszewski, JH
影响因子：
3.6
作者：
Duquette, RA;Shmygol, A;Wray, S
通讯作者：
Wray, S
影响因子：
29.4
作者：
He, CL;Burgart, L;Farrugia, G
通讯作者：
Farrugia, G
影响因子：
24.5
作者：
Lyford, GL;He, CL;Farrugia, G
通讯作者：
Farrugia, G