Noninvasive detection of macrophages in atherosclerotic lesions by computed tomography enhanced with PEGylated gold nanoparticles
Noninvasive detection of macrophages in atherosclerotic lesions by computed tomography enhanced with PEGylated gold nanoparticles
复制标题
聚乙二醇化金纳米粒子增强计算机断层扫描对动脉粥样硬化病变中巨噬细胞的无创检测
DOI：
10.2147/ijn.s72819
复制
发表时间：
2014-12
期刊：
影响因子：
--
通讯作者：
Lu Xinwu
中科院分区：
文献类型：
--
作者：
Qin Jinbao;Peng Chen;Zhao Binghui;Ye Kaichuang;Yuan Fukang;Peng Zhiyou;Yang Xinrui;Huang Lijia;Jiang Mier;Zhao Qinghua;Tang Guangyu;Lu Xinwu
巨噬细胞在动脉粥样硬化（AS）的进展中变得越来越重要。巨噬细胞分子成像可以提高AS的检测和表征。在这项研究中，树突状包裹的金纳米颗粒（Au DENPs）与聚乙二醇（PEG）和异硫氰酸荧光素（FI）涂层被设计、测试并应用于动脉粥样硬化病变中巨噬细胞的增强计算机断层扫描（CT）成像造影剂。细胞计数试剂盒-8、荧光显微镜、银染色和透射电镜显示，fi功能化的Au DENPs在高浓度（3.0 μM）下无细胞毒性，可被体外小鼠巨噬细胞有效吸收。这些纳米颗粒被给予载脂蛋白E敲除小鼠作为as模型，这表明，在活体动物中，使用micro-CT可以无创地、动态地三维跟踪动脉粥样硬化区域的巨噬细胞负荷。我们的研究结果表明，设计的聚乙二醇化金纳米颗粒是很有希望用于动脉粥样硬化病变巨噬细胞CT成像的生物相容性纳米探针，并将为AS和其他相关炎症疾病的病理生理学提供新的见解。
Macrophages are becoming increasingly significant in the progression of atherosclerosis (AS). Molecular imaging of macrophages may improve the detection and characterization of AS. In this study, dendrimer-entrapped gold nanoparticles (Au DENPs) with polyethylene glycol (PEG) and fluorescein isothiocyanate (FI) coatings were designed, tested, and applied as contrast agents for the enhanced computed tomography (CT) imaging of macrophages in atherosclerotic lesions. Cell counting kit-8 assay, fluorescence microscopy, silver staining, and transmission electron microscopy revealed that the FI-functionalized Au DENPs are noncytotoxic at high concentrations (3.0 μM) and can be efficiently taken up by murine macrophages in vitro. These nanoparticles were administered to apolipoprotein E knockout mice as AS models, which demonstrated that the macrophage burden in atherosclerotic areas can be tracked noninvasively and dynamically three-dimensionally in live animals using micro-CT. Our findings suggest that the designed PEGylated gold nanoparticles are promising biocompatible nanoprobes for the CT imaging of macrophages in atherosclerotic lesions and will provide new insights into the pathophysiology of AS and other concerned inflammatory diseases.
登录
查看更多内容
影响因子：
20.1
作者：
Quillard T;Libby P
通讯作者：
Libby P
影响因子：
15.9
作者：
Potteaux, Stephane;Gautier, Emmanuel L.;Randolph, Gwendalyn J.
通讯作者：
Randolph, Gwendalyn J.
影响因子：
13.3
作者：
Shi, Xiangyang;Wang, Suhe;Baker, James R., Jr.
通讯作者：
Baker, James R., Jr.
影响因子：
14
作者：
Qin, Jinbao;Li, Kangan;Lu, Xinwu
通讯作者：
Lu, Xinwu
DOI：
10.1016/j.yvas.2011.02.004
发表时间：
2011
期刊：
Yearbook of Vascular Surgery
影响因子：
--
作者：
G. Moneta
通讯作者：
G. Moneta