Lateral flow microarrays: a novel platform for rapid nucleic acid detection based on miniaturized lateral flow chromatography.
Lateral flow microarrays: a novel platform for rapid nucleic acid detection based on miniaturized lateral flow chromatography.
复制标题
DOI：
10.1093/nar/gkm269
复制
发表时间：
2007
影响因子：
14.9
通讯作者：
Cary RB
中科院分区：
文献类型：
--
作者：
Carter DJ;Cary RB
广泛使用的核酸分析不太适合现场部署，因为在现场部署时，获得实验室仪器的机会有限或无法获得。对现场可部署的核酸检测的需求要求在不牺牲信息容量或灵敏度的情况下使用廉价、方便的系统。在这里，我们描述了一种新型的微阵列平台，能够快速、灵敏地检测核酸，而不需要专门的仪器。该方法基于一种利用杂交介导靶向捕获的微型侧向流动装置。与传统的横向流动装置相比，横向流动核酸检测的小型化提供了多方面的优势。10微升的样品体积减少了试剂消耗和产量分析物检测时间，不包括样品制备和放大，同时提供亚毫微摩尔灵敏度的<；120 S。此外，微阵列技术的使用增加了侧向流动的潜在信息容量。与基于杂交的检测方案相结合，横向流动微阵列(LFM)实现了序列特异性检测，为适合于医疗保健点和其他现场应用的大范围分析打开了高度多元化的实施之门。LFM系统是使用等温放大策略来检测炭疽芽孢杆菌，炭疽的病原体。在没有热循环硬件或荧光检测系统的情况下，仅从两个炭疽杆菌细胞中检测到了RNA。
Widely used nucleic acid assays are poorly suited for field deployment where access to laboratory instrumentation is limited or unavailable. The need for field deployable nucleic acid detection demands inexpensive, facile systems without sacrificing information capacity or sensitivity. Here we describe a novel microarray platform capable of rapid, sensitive nucleic acid detection without specialized instrumentation. The approach is based on a miniaturized lateral flow device that makes use of hybridization-mediated target capture. The miniaturization of lateral flow nucleic acid detection provides multiple advantages over traditional lateral flow devices. Ten-microliter sample volumes reduce reagent consumption and yield analyte detection times, excluding sample preparation and amplification, of <120 s while providing sub-femtomole sensitivity. Moreover, the use of microarray technology increases the potential information capacity of lateral flow. Coupled with a hybridization-based detection scheme, the lateral flow microarray (LFM) enables sequence-specific detection, opening the door to highly multiplexed implementations for broad-range assays well suited for point-of-care and other field applications. The LFM system is demonstrated using an isothermal amplification strategy for detection of Bacillus anthracis, the etiologic agent of anthrax. RNA from as few as two B. anthracis cells was detected without thermocycling hardware or fluorescence detection systems.
登录
查看更多内容
影响因子：
4.4
作者：
Hill, KK;Ticknor, LO;Jackson, PJ
通讯作者：
Jackson, PJ
影响因子：
9.4
作者：
Fong, WK;Modrusan, Z;Bekkaoui, F
通讯作者：
Bekkaoui, F
影响因子：
9.4
作者：
Easterday, WR;Van Ert, MN;Keim, P
通讯作者：
Keim, P
影响因子：
120.1
作者：
Koch, WH
通讯作者：
Koch, WH
影响因子：
9.4
作者：
Dineva, MA;Candotti, D;Lee, H
通讯作者：
Lee, H