Comparative Determination of Cytotoxicity of Sub-10 nm Copper Nanoparticles to Prokaryotic and Eukaryotic Systems.
Comparative Determination of Cytotoxicity of Sub-10 nm Copper Nanoparticles to Prokaryotic and Eukaryotic Systems.
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DOI：
10.1021/acsami.0c11052
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发表时间：
2020-11-11
影响因子：
9.5
通讯作者：
Zhang K
中科院分区：
文献类型：
--
作者：
Skeeters SS;Rosu AC;Divyanshi;Yang J;Zhang K
铜纳米粒子具有抗菌活性，但其对真核系统的毒性了解较少。在这里，我们进行了一项比较研究，以确定亚10 nm铜纳米粒子的生物相容性和细胞毒性的各种生物系统，包括原核细胞（大肠杆菌），酵母，哺乳动物细胞系（HEK 293 T，PC 12），和斑马鱼胚胎。我们通过探测细胞生长、活力以及胚胎学特征来确定铜纳米颗粒诱导细胞死亡浓度的承载阈值。为了排除残留反应物的部分毒性效应，我们开发了一种使用琼脂糖凝胶电泳的纯化方法。纯化的CuONP溶液在18 h的处理期间分别在170和122.5ppm（w/w）下抑制细菌生长并引起真核细胞死亡。CuONP在85 ppm时显著降低斑马鱼胚胎视网膜色素上皮的色素沉着。CuONP对真核细胞的毒性可能是由其诱导的氧化应激引起的。这一结果表明，小的铜纳米颗粒在原核和真核系统中都发挥细胞毒性，因此，考虑到铜纳米颗粒在临床环境中的潜在用途，应谨慎避免铜纳米颗粒与人体组织直接接触。
Copper nanoparticles demonstrate antibacterial activity but their toxicity to eukaryotic systems is less understood. Here, we carried out a comparative study to determine the biocompatibility and cytotoxicity of sub-10 nm copper nanoparticles to a variety of biological systems, including prokaryotic cells (Escherichia coli), yeast, mammalian cell lines (HEK293T, PC12), and zebrafish embryos. We determined the bearing threshold for the cell-death-inducing concentration of copper nanoparticles by probing cell growth, viability, as well as embryological features. To exclude the partial toxicity effect from the remnant reactants, we developed a purification approach using agarose gel electrophoresis. Purified CuONP solution inhibits bacterial growth and causes eukaryotic cell death at 170 and 122.5 ppm (w/w) during the 18 h of treatment, respectively. CuONP significantly reduces the pigmentation of retina pigmented epithelium of zebrafish embryos at 85 ppm. The cytotoxicity to eukaryotic cells could arise from the oxidative stress induced by CuONP. This result suggests that small copper nanoparticles exert cytotoxicity in both prokaryotic and eukaryotic systems, and therefore, caution should be used to avoid direct contact of copper nanoparticles to human tissues considering the potential use of copper nanoparticles in the clinical setting.
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DOI：
10.3390/s130607926
发表时间：
2013-06-20
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Ibupoto ZH;Khun K;Beni V;Liu X;Willander M
通讯作者：
Willander M
影响因子：
0.8
作者：
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通讯作者：
Glushchenko, N. N.
影响因子：
3.5
作者：
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通讯作者：
Basu, Tarakdas
DOI：
10.1016/j.bbrc.2019.07.110
发表时间：
2019-10-01
影响因子：
3.1
作者：
Alizadeh, Sanaz;Seyedalipour, Bagher;Aghdami, Nasser
通讯作者：
Aghdami, Nasser
影响因子：
4.5
作者：
Chahdoura, Faouzi;Pradel, Christian;Gomez, Montserrat
通讯作者：
Gomez, Montserrat