From kirigami to three-dimensional paper-based micro-analytical device: cut-and-paste fabrication and mobile app quantitation.
From kirigami to three-dimensional paper-based micro-analytical device: cut-and-paste fabrication and mobile app quantitation.
复制标题
从剪纸到三维纸基微分析设备：剪切粘贴制造和移动应用程序定量
作者：
目前，定量化学分析通常是昂贵的、仪器依赖的、耗时的，这限制了它在偏远地区和资源有限地区的实施。受古代剪纸艺术（kirigami）的启发，我们在此介绍了一种新的剪切粘贴方案来制作适合现场定量分析应用的3D微流体纸基分析装置（μ pad）。该器件的制备快速、简单，并且独立于任何光刻设备或掩模。特别设计的反应“通道”是从一张滤纸上预先剪下来的，然后组装回硅化的超疏水纸垫。该装置的不同层使用化学惰性粘合剂喷雾进行组装。该装置具有高效率的液体处理（比传统方法快60倍），而且特别便宜。除了台式制造优势之外，结合用于比色分析的定制移动应用程序，我们能够使用剪切粘贴μ pad（即kpad）量化各种水样中的代表性环境污染物（即Cr（vi）和亚硝酸盐离子的量）。它们的检出限（Cr（vi）为0.7 μg mL−1，亚硝酸盐离子为0.4 μg mL−1）与传统分光光度法相当，这证实了kpad在现场环境/卫生监测和食品毒素预筛选方面的潜力。
Nowadays quantitative chemical analysis is usually costly, instrument-dependent, and time-consuming, which limits its implementation for remote locations and resource-limited regions. Inspired by the ancient papercutting art (kirigami), we herein introduce a novel cut-and-paste protocol to fabricate 3D microfluidic paper-based analytical devices (μPADs) that are suitable for on-site quantitative assay applications. The preparation of the device is fast, simple, and independent of any lithographic devices or masks. Particularly designed reaction “channels” were pre-cut from a piece of filter paper, then assembled back to the silanized, superhydrophobic paper pads. The different layers of the device were assembled using a chemically-inert adhesive spray. The fabricated device has high efficiency of liquid handling (up to 60 times faster than conventional methods) and it is particularly inexpensive. Beyond the benchtop fabrication advantage, in conjunction with a custom mobile app developed for colorimetric analysis, we were able to quantify representative environmental contaminants (i.e., the amount of Cr(vi) and nitrite ions) in various water samples with the cut-and-paste μPADs (namely kPADs). Their detection limits (0.7 μg mL−1 for Cr(vi) and 0.4 μg mL−1 for nitrite ions, respectively) are comparable with conventional spectrophotometric methods, which confirm the potential of kPADs for on-site environmental/sanitary monitoring and food toxin pre-screening.
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影响因子：
6.2
作者：
Bhakta SA;Borba R;Taba M Jr;Garcia CD;Carrilho E
通讯作者：
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12.6
作者：
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影响因子：
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影响因子：
2.7
作者：
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通讯作者：
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