Direct measurement of the direction-dependent mechanical behaviour of skeletal muscle extracellular matrix.
Direct measurement of the direction-dependent mechanical behaviour of skeletal muscle extracellular matrix.
复制标题
直接测量骨骼肌细胞外基质的方向依赖性机械行为
DOI：
10.1016/j.actbio.2020.12.050
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发表时间：
2021
影响因子：
9.7
通讯作者：
M. Böl
中科院分区：
文献类型：
--
作者：
S. Kohn;K. Leichsenring;R. Kuravi. A E. Ehret;M. Böl
本文报告了第一个关于骨骼肌分离的肌内和肌周细胞外基质各向异性机械特性的综合数据集，并提出了用于制备和测试样品的相应方案。特别是，用烧碱溶液实现猪骨骼肌的脱细胞，并根据压缩和拉伸测试定义机械参数，以确定最佳处理时间，使肌纤维溶解，而细胞外基质基本保持完整和机械功能。在18小时左右，发现并通过组织学证实了一个时间窗口，其中进行轴向拉伸实验来表征细胞外基质样品的方向依赖性机械响应，并研究横向预压缩的效果。通过改变单个标量因子可以大大减少实验应激反应中典型的大变异性，这归因于组织内细胞外基质分数的变化。虽然文献中越来越多地提供有关完整肌肉组织和单肌纤维机械特性的实验结果，但缺乏有关骨骼肌胶原成分特性的信息。目前的工作旨在缩小这一差距，从而有助于提高对骨骼肌组织力学的理解，并为相应的构成和计算模型的开发提供缺失的信息。
This paper reports the first comprehensive data set on the anisotropic mechanical properties of isolated endo- and perimysial extracellular matrix of skeletal muscle, and presents the corresponding protocols for preparing and testing the samples. In particular, decellularisation of porcine skeletal muscle is achieved with caustic soda solution, and mechanical parameters are defined based on compressive and tensile testing in order to identify the optimal treatment time such that muscle fibres are dissolved whereas the extracellular matrix remains largely intact and mechanically functional. At around 18 h, a time window was found and confirmed by histology, in which axial tensile experiments were performed to characterise the direction-dependent mechanical response of the extracellular matrix samples, and the effect of lateral pre-compression was studied. The typical, large variability in the experimental stress response could be largely reduced by varying a single scalar factor, which was attributed to the variation of the fraction of extracellular matrix within the tissue. While experimental results on the mechanical properties of intact muscle tissue and single muscle fibres are increasingly available in literature, there is a lack of information on the properties of the collagenous components of skeletal muscle. The present work aims at closing this gap and thus contributes to an improved understanding of the mechanics of skeletal muscle tissue and provides a missing piece of information for the development of corresponding constitutive and computational models.
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DOI：
10.1115/1.4028088
发表时间：
2014-11-01
影响因子：
1.7
作者：
Pietsch, Renee;Wheatley, Benjamin B.;Williams, Lakiesha N.
通讯作者：
Williams, Lakiesha N.
影响因子：
4.4
作者：
Yoo L;Kim H;Gupta V;Demer JL
通讯作者：
Demer JL
将宏观组织应变转移到变形半月板中的微观细胞区域。
影响因子：
3.4
作者：
Upton,MaureenL;Gilchrist,ChristopherL;Guilak,Farshid;Setton,LoriA
通讯作者：
Setton,LoriA
受小拉伸应变作用的死后人体肌肉的实验表征以及简单超粘弹性模型的应用
DOI：
10.1177/0954411914555422
发表时间：
2014
期刊：
Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine
影响因子：
--
作者：
L. Gras;S. Laporte;P. Viot;D. Mitton
通讯作者：
D. Mitton
DOI：
10.1016/j.jmbbm.2009.03.004
发表时间：
2010-01
影响因子：
3.9
作者：
Morrow, Duane A.;Donahue, Tammy L. Haut;Odegard, Gregory M.;Kaufman, Kenton R.
通讯作者：
Kaufman, Kenton R.