Transcriptional analysis of a whole-body form of long-term habituation in Aplysia californica.
Transcriptional analysis of a whole-body form of long-term habituation in Aplysia californica.
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DOI：
10.1101/lm.036970.114
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发表时间：
2014-01
期刊：
影响因子：
--
通讯作者：
Calin-Jageman RJ
中科院分区：
文献类型：
--
作者：
Holmes G;Herdegen S;Schuon J;Cyriac A;Lass J;Conte C;Calin-Jageman IE;Calin-Jageman RJ
习惯化是最简单的学习形式，但我们对编码长期习惯化记忆的转录机制知之甚少。一个关键的障碍是习惯化是相对刺激特异性的，因此在小的神经元集合中编码，为转录分析提供了差的信噪比。为了克服这一障碍，我们已经开发出一种方案，用于产生全身长期习惯的虹吸管撤回反射（SWR）的Aaplasia californica。具体来说，我们构建了一个计算机控制的刷牙装置，以定期在整个背表面的Aaplasia低强度的触觉刺激。我们发现，3天的训练（10轮刺激/天;每轮= 15分钟刷牙，10秒ISI;轮之间休息15分钟）产生的习惯与几个特点有利于机械调查。首先，习惯化是普遍的，无论反射是由头部、尾部或虹吸管的触觉刺激引起的，SWR持续时间都会减少。第二，长期习惯化对训练模式很敏感，只有当刷牙时间间隔超过3d而不是集中在一个单一的时间段时才会发生。使用定制设计的微阵列和定量PCR，我们表明，长期的习惯性产生长期上调的一个明显的Aaplasia同源的cornichon，谷氨酸受体贩运的重要蛋白质。我们的训练范式为表征长期习惯化记忆的转录机制提供了一个有希望的起点。
Habituation is the simplest form of learning, but we know little about the transcriptional mechanisms that encode long-term habituation memory. A key obstacle is that habituation is relatively stimulus-specific and is thus encoded in small sets of neurons, providing poor signal/noise ratios for transcriptional analysis. To overcome this obstacle, we have developed a protocol for producing whole-body long-term habituation of the siphon-withdrawal reflex (SWR) of Aplysia californica. Specifically, we constructed a computer-controlled brushing apparatus to apply low-intensity tactile stimulation over the entire dorsal surface of Aplysia at regular intervals. We found that 3 d of training (10 rounds of stimulation/day; each round = 15 min brushing at a 10-sec ISI; 15-min rest between rounds) produces habituation with several characteristics favorable for mechanistic investigation. First, habituation is widespread, with SWR durations reduced whether the reflex is evoked by tactile stimulation to the head, tail, or the siphon. Second, long-term habituation is sensitive to the pattern of training, occurring only when brushing sessions are spaced out over 3 d rather than massed into a single session. Using a custom-designed microarray and quantitative PCR, we show that long-term habituation produces long-term up-regulation of an apparent Aplysia homolog of cornichon, a protein important for glutamate receptor trafficking. Our training paradigm provides a promising starting point for characterizing the transcriptional mechanisms of long-term habituation memory.
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影响因子：
2.5
作者：
Dong, S.;Clayton, D. F.
通讯作者：
Clayton, D. F.
影响因子：
2.5
作者：
BYRNE, J;CASTELLU.V;KANDEL, ER
通讯作者：
KANDEL, ER
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
16.2
作者：
Brockie PJ;Jensen M;Mellem JE;Jensen E;Yamasaki T;Wang R;Maxfield D;Thacker C;Hoerndli F;Dunn PJ;Tomita S;Madsen DM;Maricq AV
通讯作者：
Maricq AV
影响因子：
2.5
作者：
Freudenthal, R;Locatelli, F;Romano, A
通讯作者：
Romano, A